Division of Infectious Animal Disease Research, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki, Japan.
Oita Prefectural Oita Livestock Hygiene Service Center, Oita, Japan.
J Vet Med Sci. 2024 Sep 1;86(9):925-929. doi: 10.1292/jvms.24-0086. Epub 2024 Jul 12.
Clostridium sp. was detected in the organs of a cow with black watery diarrhea in Japan. Results identifying this species were inconsistent; Clostridium novyi type A infection was suggested by PCR assay targeting Clostridium fliC region (fliC-multiplex PCR), while 16S rRNA gene sequencing identified the isolated bacteria as Clostridium massiliodielmoense. Sequencing of fliC-multiplex PCR products from the isolates revealed the presence of fliC region in C. massiliodielmoense, which had 92.7% nucleotide similarity to that of C. novyi type A JCM 1406, leading to the false positive detection of C. novyi by the PCR. This is the first C. massiliodielmoense isolation from clinical specimens, suggesting the need for further research on its pathogenicity and improvement in fliC-multiplex PCR.
在日本，一种具有黑色水样腹泻症状的奶牛的器官中检测到了梭菌属。鉴定该物种的结果不一致；针对梭菌 fliC 区（fliC-多重 PCR）的 PCR 检测提示存在 A 型诺维梭菌感染，而 16S rRNA 基因测序则将分离出的细菌鉴定为马氏梭菌。从分离株中 fliC-多重 PCR 产物的测序显示，马氏梭菌存在 fliC 区，与 A 型诺维梭菌 JCM 1406 的核苷酸相似性为 92.7%，导致 PCR 对 C. novyi 的假阳性检测。这是首次从临床标本中分离出马氏梭菌，提示需要进一步研究其致病性并改进 fliC-多重 PCR。
