Hara Shunsuke, Shirasuna Koumei, Iwata Hisataka
Department of Animal Science, Tokyo University of Agriculture, Tokyo 156-8502, Japan.
J Reprod Dev. 2024 Oct 1;70(5):303-308. doi: 10.1262/jrd.2024-017. Epub 2024 Jul 13.
Gellan gum (GG) is a soft, tractable, and natural polysaccharide substrate used for cell incubation. In this study, we examined the effects of GG on porcine oocyte maturation. Cumulus cells and oocyte complexes (COCs) were collected from slaughterhouse-derived porcine ovaries and cultured on plastic plates containing 0.05% or 0.1% GG gels. The 0.1% GG gel improved the maturation rate and quality of blastocysts, as determined by the total cell number and the rate of abnormally condensed nuclei. GG gels have antioxidant abilities and oocytes cultured on GG gels (0.05% and 0.1%) have reduced reactive oxygen species (ROS) content. Furthermore, GG gels (0.05% and 0.1%) increased F-actin formation, whereas treatment of oocytes with HO reduced F-actin levels. GG gels increased the ATP content in oocytes but did not affect the mitochondrial DNA copy number or mitochondrial membrane potential. In addition, the medium cultured on 0.05% GG increased the glucose consumption of COCs. In conclusion, GG gel reduced ROS content, increased energy content, and improved subsequent embryonic development in pigs.
结冷胶（GG）是一种用于细胞培养的柔软、易处理的天然多糖底物。在本研究中，我们检测了GG对猪卵母细胞成熟的影响。从屠宰场获得的猪卵巢中采集卵丘细胞-卵母细胞复合体（COCs），并在含有0.05%或0.1% GG凝胶的塑料培养板上进行培养。通过总细胞数和异常浓缩核的比例确定，0.1% GG凝胶提高了囊胚的成熟率和质量。GG凝胶具有抗氧化能力，在GG凝胶（0.05%和0.1%）上培养的卵母细胞活性氧（ROS）含量降低。此外，GG凝胶（0.05%和0.1%）增加了F-肌动蛋白的形成，而用HO处理卵母细胞会降低F-肌动蛋白水平。GG凝胶增加了卵母细胞中的ATP含量，但不影响线粒体DNA拷贝数或线粒体膜电位。此外，在0.05% GG上培养的培养基增加了COCs的葡萄糖消耗。总之，GG凝胶降低了ROS含量，增加了能量含量，并改善了猪随后的胚胎发育。
