Pacelli Settimio, Paolicelli Patrizia, Petralito Stefania, Subham Siddharth, Gilmore Drake, Varani Gabriele, Yang Guang, Lin Dong, Casadei Maria Antonietta, Paul Arghya
Department of Chemical and Petroleum Engineering, BioIntel Research Laboratory, University of Kansas, Lawrence, Kansas 66045, United States.
Department of Drug Chemistry and Technologies, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy.
ACS Appl Bio Mater. 2020 Feb 17;3(2):945-951. doi: 10.1021/acsabm.9b00989. Epub 2020 Jan 13.
Gellan gum-based hydrogels display limited cell adhesion ability due to the absence of cell-anchorage points usually present in proteins found in the extracellular matrix (ECM). This issue limits their use in the biomedical field as scaffolds to promote tissue repair. Our work addresses this challenge by investigating the use of polydopamine (pDA) as a bioactive layer to improve the surface and biological properties of gellan gum-based hydrogels cross-linked using carbodiimide chemistry. Upon treatment with a pDA layer, the hydrogel displayed an increase in wettability and swelling properties. This change in physical properties had a direct impact on the biological properties of the scaffolds. Precisely, human adipose-derived stem cells (hASCs) seeded on the pDA coated gellan gum hydrogels displayed larger cell area, increased proliferation rate, and enhanced gene expression of focal adhesion and cytoskeletal proteins. Overall, the findings of this research support the use of pDA coating as a possible approach to improve the biological features of gellan gum-based scaffolds and modulate stem cell morphology and proliferation.
由于缺乏通常存在于细胞外基质（ECM）蛋白质中的细胞锚定位点，结冷胶基水凝胶的细胞粘附能力有限。这个问题限制了它们在生物医学领域作为促进组织修复支架的应用。我们的工作通过研究使用聚多巴胺（pDA）作为生物活性层来改善经碳二亚胺化学交联的结冷胶基水凝胶的表面和生物学特性，解决了这一挑战。在用pDA层处理后，水凝胶的润湿性和溶胀特性有所增加。这种物理性质的变化对支架的生物学特性有直接影响。确切地说，接种在pDA包被的结冷胶水凝胶上的人脂肪来源干细胞（hASC）表现出更大的细胞面积、更高的增殖率以及粘着斑和细胞骨架蛋白基因表达的增强。总体而言，本研究结果支持使用pDA涂层作为一种可能的方法来改善结冷胶基支架的生物学特性，并调节干细胞的形态和增殖。
