Tian Chunyan, Li Kun, Chu Fuhuan, Wei Qiujiang, Xu Shiqi, Qiang Linhui, Gou Xinrui
Department of Biomedical Engineering, Chengde Medical University Chengde 067000 Hebei China
Hebei International Research Center for Medical-Engineering, Chengde Medical University Chengde 067000 Hebei China.
RSC Adv. 2024 Jul 15;14(31):22420-22433. doi: 10.1039/d4ra04047c. eCollection 2024 Jul 12.
Traditional bone tissue engineering techniques require the extraction and proliferation of seed cells, followed by prolonged culture to form bone tissue constructs. In contrast, mineralization bone tissue engineering utilizes alkaline phosphatase within the body's microenvironment to induce scaffold mineralization. This approach promotes further proliferation and differentiation of osteoblasts and the formation of bone tissue constructs, thereby simplifying the traditional bone tissue engineering process. This study uses electrospinning technology to prepare a novel biologically active scaffold for bone tissue engineering using poly(lactic--glycolic acid) (PLGA) and calcium glycerophosphate. The morphology and composition of the scaffolds were characterized using SEM, EDS, and XRD, revealing well-defined fibrous structures and the successful incorporation of calcium glycerophosphate into the PLGA fibers. simulation of the bone microenvironment using alkaline phosphatase effectively catalyzed the mineralization of calcium glycerophosphate within the scaffold. SEM observations showed substantial mineral aggregation on the surface of the fibrous membranes, and XRD characterization confirmed that the diffraction peaks of the minerals correspond to hydroxyapatite. The cytotoxicity, cell proliferation, and osteogenic differentiation assessments on MC3T3-E1 pre-osteoblasts cultured on the prepared scaffolds indicate that the scaffolds are non-toxic to cells and possess good osteogenic differentiation ability, enabling mineralization. This suggests that the scaffolds have broad prospects for application in bone defect repair.
传统的骨组织工程技术需要提取和增殖种子细胞，然后进行长时间培养以形成骨组织构建体。相比之下，矿化骨组织工程利用体内微环境中的碱性磷酸酶来诱导支架矿化。这种方法促进成骨细胞的进一步增殖和分化以及骨组织构建体的形成，从而简化了传统的骨组织工程过程。本研究采用静电纺丝技术，以聚乳酸-乙醇酸共聚物（PLGA）和甘油磷酸钙制备了一种新型的用于骨组织工程的生物活性支架。使用扫描电子显微镜（SEM）、能谱仪（EDS）和X射线衍射仪（XRD）对支架的形态和组成进行了表征，结果显示出明确的纤维结构，并且甘油磷酸钙成功地掺入到PLGA纤维中。使用碱性磷酸酶模拟骨微环境有效地催化了支架内甘油磷酸钙的矿化。SEM观察显示纤维膜表面有大量矿物质聚集，XRD表征证实矿物质的衍射峰与羟基磷灰石相对应。对在制备的支架上培养的MC3T3-E1前成骨细胞进行的细胞毒性、细胞增殖和成骨分化评估表明，该支架对细胞无毒，具有良好的成骨分化能力，能够矿化。这表明该支架在骨缺损修复方面具有广阔的应用前景。
