Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.
Department of Medicinal Chemistry and Pharmacology, University of Science and Technology, Daejeon 34113, Republic of Korea.
J Agric Food Chem. 2024 Jul 24;72(29):16359-16367. doi: 10.1021/acs.jafc.4c04706. Epub 2024 Jul 16.
In our screening program for natural products that are effective in controlling plant diseases, we found that the culture filtrate of SFC20160907-M11 effectively suppressed the development of tomato late blight disease caused by . Using a bioassay-guided fractionation of antioomycete activity, 12 active compounds (-) were obtained from an ethyl acetate extract of the culture filtrate. Chemical structures of five new compounds - were determined by the extensive analyses of nuclear magnetic resonance (NMR), high resolution mass spectrometry (HRMS), and circular dichroism (CD) data. Interestingly, mycosporulonol () and botrallin () completely inhibited the growth of at concentrations of 8 and 16 μg/mL, respectively. Furthermore, the spray treatment of and (500 μg/mL) successfully protected tomato seedlings against with disease control values of 92%. Taken together, these results suggest that the culture filtrates of SFC20160907-M11 and their bioactive metabolites can be used as new antioomycete agents for Phytophthora late blight control.
在我们针对具有防治植物病害效果的天然产物的筛选计划中，发现 SFC20160907-M11 的培养滤液能有效抑制 引起的番茄晚疫病的发展。通过抗真菌活性的生物测定指导的分级分离，从培养滤液的乙酸乙酯提取物中获得了 12 种活性化合物 (-)。通过对核磁共振 (NMR)、高分辨率质谱 (HRMS) 和圆二色性 (CD) 数据的广泛分析，确定了五个新化合物 - 的化学结构。有趣的是，麦角固醇（）和 botrallin（）分别在 8 和 16 μg/mL 的浓度下完全抑制了 的生长。此外，（500 μg/mL）的喷雾处理成功地保护了番茄幼苗免受 的侵害，病害防治率达到 92%。综上所述，这些结果表明 SFC20160907-M11 的培养滤液及其生物活性代谢物可用作防治卵菌晚疫病的新型抗真菌剂。
