Park Je-Hyun, Han Jae Woo, Kim Bomin, Park Sang Un, Choi Gyung Ja, Kim Hun
Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon, South Korea.
Department of Crop Science, Chungnam National University, Daejeon, South Korea.
Pest Manag Sci. 2025 Sep;81(9):5052-5061. doi: 10.1002/ps.8859. Epub 2025 May 6.
In the search for new natural resources with antifungal activity, the culture filtrate of the marine-derive fungus Paraboeremia adianticola SFC20150402-M24 showed excellent activity for controlling rice blast and tomato late blight diseases. This study aimed to (i) identify antifungal substances from the P. adianticola culture filtrate using chromatography and spectroscopy techniques and (ii) investigate the in vitro and in vivo antifungal activities of the isolated compound, along with its underlying antifungal mechanisms.
Based on chromatographic and spectroscopic analyses, vulculic acid was isolated and identified as a principal antifungal compound of P. adianticola SFC20150402-M24. Among the tested eight fungal pathogens, vulculic acid completely inhibited the growth of Magnaporthe oryzae and Phytophthora infestans at concentrations of 6.3 and 200 μg mL, respectively. This inhibition resulted in effective control of rice blast and tomato late blight. Furthermore, given that vulculic acid was more effective in inhibiting spore germination than the mycelial growth of M. oryzae, our results showed that vulculic acid has an effect on mitochondrial respiration with the downregulation of SdhC and Cox7A genes and inhibitory activity for electron transporter chain complexes II, III, and IV.
These findings highlight P. adianticola SFC20150402-M24 and its production of vulculic acid as a valuable biological control agent for rice blast and tomato late blight. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
在寻找具有抗真菌活性的新自然资源的过程中，海洋来源真菌阿迪安蒂科拉拟博发菌（Paraboeremia adianticola）SFC20150402 - M24的培养滤液对控制稻瘟病和番茄晚疫病表现出优异活性。本研究旨在：（i）使用色谱和光谱技术从阿迪安蒂科拉拟博发菌的培养滤液中鉴定抗真菌物质；（ii）研究分离出的化合物的体外和体内抗真菌活性及其潜在的抗真菌机制。
基于色谱和光谱分析，分离并鉴定出戊曲酸是阿迪安蒂科拉拟博发菌SFC20150402 - M24的主要抗真菌化合物。在测试的八种真菌病原体中，戊曲酸分别在浓度为6.3和200μg/mL时完全抑制了稻瘟病菌（Magnaporthe oryzae）和致病疫霉（Phytophthora infestans）的生长。这种抑制作用有效控制了稻瘟病和番茄晚疫病。此外，鉴于戊曲酸对稻瘟病菌孢子萌发的抑制作用比对其菌丝生长更有效，我们的结果表明戊曲酸对线粒体呼吸有影响，可下调SdhC和Cox7A基因，并对电子传递链复合物II、III和IV具有抑制活性。
这些发现突出了阿迪安蒂科拉拟博发菌SFC20150402 - M24及其产生的戊曲酸作为稻瘟病和番茄晚疫病的有价值生物防治剂的作用。© 2025作者。由John Wiley & Sons Ltd代表化学工业协会出版的《害虫管理科学》。
