Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.
Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy, Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia.
Luminescence. 2024 Jul;39(7):e4814. doi: 10.1002/bio.4814.
Olopatadine (OLP) is widely utilized as an effective antihistaminic drug for alleviating ocular itching associated with allergic conjunctivitis. With its frequent usage in pharmacies, there arises a pressing need for a cost-effective, easily implementable, environmentally sustainable detection method with high sensitivity. This study presents a novel signal-on fluorimetric method for detecting OLP in both its pure form and aqueous humor. The proposed approach depends on enhancing the weak intrinsic fluorescence emission of OLP, achieving a remarkable increase of up to 680% compared to its intrinsic fluorescence. This enhancement is achieved by forming micelles around protonated OLP using an acetate buffer (pH 3.6) and incorporating a solution of sodium dodecyl sulfate (SDS) surfactant. A strong correlation (R = 0.9996) is observed between the concentration of OLP and fluorescence intensities ranging from 1.0 to 100.0 ng mL with a limit of detection of 0.22 ng mL. This described method is successfully employed for quantifying OLP in both its powder form and pharmaceutical eye drops. Furthermore, it demonstrates robust performance in determining OLP in artificial aqueous humor with a percentage recovery of 99.05 ± 1.51, with minimal interference from matrix interferents. Moreover, the greenness of the described method was evaluated.
奥洛他定（OLP）作为一种有效的抗组胺药物，广泛用于缓解过敏性结膜炎相关的眼部瘙痒。由于其在药店中的频繁使用，因此迫切需要一种经济有效、易于实施、对环境可持续且具有高灵敏度的检测方法。本研究提出了一种用于检测 OLP 纯品和房水中 OLP 的新型荧光开启方法。该方法依赖于增强 OLP 的弱固有荧光发射，与固有荧光相比，其荧光强度增强高达 680%。这种增强是通过使用醋酸盐缓冲液（pH 3.6）在质子化的 OLP 周围形成胶束并加入十二烷基硫酸钠（SDS）表面活性剂溶液来实现的。在 1.0 至 100.0ng mL 的浓度范围内，观察到 OLP 的浓度与荧光强度之间存在很强的相关性（R = 0.9996），检测限为 0.22ng mL。该方法成功地用于检测 OLP 粉末和眼药水中的 OLP。此外，它在测定人工房水中的 OLP 时表现出良好的性能，回收率为 99.05 ± 1.51%，基质干扰物的干扰最小。此外，还对所描述的方法的绿色性进行了评估。
