Suraci D, Baria E, Tirloni L, Lagarto J L, Buccianti S, Agostini C, Pillozzi S, Antonuzzo L, Taddei A, Cicchi R
European Laboratory for Non-linear Spectroscopy (LENS), Sesto Fiorentino, Italy.
National Institute of Optics, National Research Council (CNR-INO), Florence, Italy.
J Biophotonics. 2024 Jul 16:e202400122. doi: 10.1002/jbio.202400122.
Autofluorescence spectroscopy has emerged in recent years as a powerful tool to report label-free contrast between normal and diseased tissues, both in vivo and ex-vivo. We report the application of an instrument employing an optical fiber probe and capable of performing real-time autofluorescence lifetime imaging at a macroscopic scale, under bright background conditions. We validate and demonstrate the practicality of this technology to discriminate healthy against neoplastic tissue in freshly excised tumor biopsies. The capability of delineating tumor margins through processing the fluorescence decays in the phasors domain was demonstrated on four different types of cancer, highlighting the broad range of potential clinical applications for the proposed approach. The presented results suggest that our autofluorescence lifetime imaging probe, together with phasor analysis, can offer a real-time tool to observe lifetime contrast on tissues and, thus, is a suitable candidate for improving in situ tissue diagnostics during surgery.
近年来，自体荧光光谱法已成为一种强大的工具，可在体内和体外报告正常组织与病变组织之间的无标记对比度。我们报告了一种仪器的应用，该仪器采用光纤探头，能够在明亮的背景条件下在宏观尺度上进行实时自体荧光寿命成像。我们验证并证明了该技术在新鲜切除的肿瘤活检中区分健康组织与肿瘤组织的实用性。通过在相量域中处理荧光衰减来描绘肿瘤边缘的能力在四种不同类型的癌症中得到了证明，突出了所提出方法广泛的潜在临床应用。所呈现的结果表明，我们的自体荧光寿命成像探头与相量分析一起，可以提供一个实时工具来观察组织上的寿命对比度，因此，是改善手术中原位组织诊断的合适候选者。
