Santi D V, Cunnion S O, Anderson R T, Webster R W
J Med Chem. 1979 Oct;22(10):1260-3. doi: 10.1021/jm00196a021.
N-Benzyl-D-amphetamine is a potent in vitro and in vivo inhibitor of phenylalanyl-tRNA synthetase of Escherichia coli. The concentration of this inhibitor necessary for the in vivo inhibition is approximately 100-fold greater than that necessary for inhibition of the purified enzyme. Treatment of rel+ strains of E. coli with the inhibitor results in a decreased percentage of tRNA Phe which is charged, guanosine tetraphosphate formation, cessation of RNA synthesis, and growth arrest. Evidence is presented which demonstrates that the primary and perhaps sole mode of action of N-benzyl-D-amphetamine is inhibition of phenylalanyl-tRNA synthetase.
N-苄基-D-苯丙胺是一种对大肠杆菌苯丙氨酰-tRNA合成酶具有强大体外和体内抑制作用的物质。体内抑制所需的该抑制剂浓度比抑制纯化酶所需的浓度大约高100倍。用该抑制剂处理大肠杆菌的rel+菌株会导致苯丙氨酰-tRNA负载的百分比降低、四磷酸鸟苷形成、RNA合成停止以及生长停滞。有证据表明，N-苄基-D-苯丙胺的主要作用方式（可能也是唯一作用方式）是抑制苯丙氨酰-tRNA合成酶。
