Johnson K H, Westermark P, Nilsson G, Sletten K, O'Brien T D, Hayden D W
Vet Pathol. 1985 Sep;22(5):463-8. doi: 10.1177/030098588502200505.
Utilizing islet amyloid-laden pancreatic tissues from six diabetic cats, we demonstrated substantial immunoreactivity (peroxidase-antiperoxidase technique) of the islet amyloid with antiserum to a B chain-rich insulin fraction, but no reactivity with antisera to insulin, glucagon, or somatostatin. Islet amyloid was purified from two cats and a protein unique to the diabetic and islet amyloid-laden cats was separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Immunoreactivity of this protein with antiserum to the B chain-rich insulin fraction was also shown by immunoblotting. Attempts to obtain the amino acid composition of the purified unique protein (represented by a single 25,000 dalton band on gel electrophoresis) were not successful because the amount of protein was too small. These results provide important additional evidence that an insulin-related protein is involved in the formation of islet amyloid. Our study also shows that the diabetic cat provides several advantages for the continued study of the etiopathogenic relationship of islet amyloid and diabetes mellitus.
利用来自六只糖尿病猫的富含胰岛淀粉样蛋白的胰腺组织，我们证明了胰岛淀粉样蛋白与富含B链的胰岛素组分抗血清具有显著的免疫反应性（过氧化物酶-抗过氧化物酶技术），但与胰岛素、胰高血糖素或生长抑素的抗血清无反应性。从两只猫中纯化出胰岛淀粉样蛋白，并通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳分离出糖尿病猫和富含胰岛淀粉样蛋白的猫所特有的一种蛋白质。免疫印迹也显示了这种蛋白质与富含B链的胰岛素组分抗血清的免疫反应性。由于蛋白质的量太少，未能成功获得纯化的独特蛋白质（在凝胶电泳上由一条单一的25,000道尔顿条带代表）的氨基酸组成。这些结果提供了重要的额外证据，表明一种与胰岛素相关的蛋白质参与了胰岛淀粉样蛋白的形成。我们的研究还表明，糖尿病猫为继续研究胰岛淀粉样蛋白与糖尿病的病因关系提供了几个优势。
