Department of Chemistry, Christ University, Hosur Road, Bangalore, Karnataka, 560029, India.
Department of Life Science, Christ University, Hosur Road, Bangalore, Karnataka, 560029, India.
Anal Methods. 2024 Aug 1;16(30):5263-5271. doi: 10.1039/d4ay00675e.
A fluorescent molecule, pyridine-coupled bis-anthracene (PBA), has been developed for the selective fluorescence turn-on detection of Cu. Interestingly, the ligand PBA also exhibited a red-shifted ratiometric fluorescence response in the presence of water. Thus, a ratiometric water sensor has been utilized as a selective fluorescence turn-on sensor for Cu, achieving a 10-fold enhancement in the fluorescence and quantum yield at 446 nm, with a lower detection limit of 0.358 μM and a binding constant of 1.3 × 10 M. For practical applications, sensor PBA can be used to detect Cu in various types of soils like clay soil, field soil and sand. The interaction of the PBA-Cu(II) complex with transport proteins like bovine serum albumin (BSA) and ct-DNA has been investigated through fluorescence titration experiments. Additionally, the structural optimization of PBA and the PBA-Cu(II) complex has been demonstrated by DFT, and the interaction of the PBA-Cu(II) complex with BSA and ct-DNA has been analyzed using theoretical docking studies.
一种荧光分子，吡啶偶联双蒽（PBA），已被开发用于选择性荧光开启检测 Cu。有趣的是，配体 PBA 在存在水的情况下也表现出红移的比率荧光响应。因此，比率水传感器已被用作选择性荧光开启传感器用于 Cu，在 446nm 处荧光和量子产率提高了 10 倍，检测限为 0.358μM，结合常数为 1.3×10 M。对于实际应用，传感器 PBA 可用于检测各种类型的土壤中的 Cu，如粘土、田间土壤和沙子。通过荧光滴定实验研究了 PBA-Cu(II)配合物与转运蛋白如牛血清白蛋白（BSA）和 ct-DNA 的相互作用。此外，通过 DFT 证明了 PBA 和 PBA-Cu(II)配合物的结构优化，并通过理论对接研究分析了 PBA-Cu(II)配合物与 BSA 和 ct-DNA 的相互作用。
