He Long, Wang Wenji, Ma Liman, Wang Dongguo, Long Shanshan
Department of Clinical Laboratory Medicine, Wenling First People's Hospital, Taizhou, Zhejiang, China.
School of Life Sciences, Taizhou University, Taizhou, Zhejiang, China.
Front Cell Infect Microbiol. 2024 Jul 2;14:1410921. doi: 10.3389/fcimb.2024.1410921. eCollection 2024.
The emergence of clinical strains harboring genes in the chromosome, along with the presence of two repetitive tandem core structures for and genes on a plasmid, has presented a significant clinical challenge.
In order to study the detailed genetic features of strain SC35, both the bacterial chromosome and plasmids were sequenced using Illumina and nanopore platforms. Furthermore, bioinformatics methods were employed to analyze the mobile genetic elements associated with antibiotic resistance genes.
strain SC35 was found to possess a class A beta-lactamase and demonstrated resistance to all tested antibiotics. This resistance was attributed to the presence of efflux pump genes, specifically , on the SC35 chromosome. Additionally, the SC35 plasmid p1 carried the two repetitive tandem core structures for and , as well as with , which shared overlapping structures with mobile genetic elements as In413, Tn, and Tn. Through plasmid transfer assays, it was determined that the SC35 plasmid p1 could be successfully transferred with an average conjugation frequency of 6.85 × 10.
The structure of the SC35 plasmid p1 appears to have evolved in correlation with other plasmids such as pKPC2_130119, pDD01754-2, and F4_plasmid pA. The infectious strain SC35 exhibits no susceptibility to tested antibioticst, thus effective measures should be taken to prevent the spread and epidemic of this strain.
染色体上携带基因的临床菌株的出现，以及质粒上存在针对和基因的两个重复串联核心结构，带来了重大的临床挑战。
为了研究菌株SC35的详细遗传特征，使用Illumina和纳米孔平台对细菌染色体和质粒进行测序。此外，采用生物信息学方法分析与抗生素抗性基因相关的移动遗传元件。
发现菌株SC35拥有A类β-内酰胺酶，并对所有测试抗生素表现出抗性。这种抗性归因于SC35染色体上存在外排泵基因，特别是。此外，SC35质粒p1携带针对和的两个重复串联核心结构，以及与，它们与In413、Tn和Tn等移动遗传元件具有重叠结构。通过质粒转移试验，确定SC35质粒p1可以成功转移，平均接合频率为6.85×10。
SC35质粒p1的结构似乎与其他质粒如pKPC2_130119、pDD01754-2和F4质粒pA相关进化。感染性菌株SC35对测试抗生素无敏感性，因此应采取有效措施防止该菌株的传播和流行。
