Li Xiaofei, Wang Yue, Li Xiaoyi, Kong Ligang, Díez Juan J, Wang Haibo, Zhang Daogong
Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial ENT Hospital, Shandong University, Jinan, China.
Shandong Provincial Vertigo & Dizziness Medical Center, Jinan, China.
Gland Surg. 2024 Jun 30;13(6):999-1015. doi: 10.21037/gs-24-157. Epub 2024 Jun 27.
There have been studies on the role of sperm-associated antigen 6 ) in cytoskeleton formation and growth cone stability, but it is also unknown how spag6 affect tumor growth and development. The aim of this study was to clarify the role of in pan-cancer, with some findings about thyroid carcinoma (THCA) validated through experiments.
We examined the role of in pan-cancer, with the data being collected from databases. Further analysis was conducted to assess its correlations with prognosis, gene heterogeneity, stemness, and tumor immunity. The interacting proteins of were also identified, and gene ontology enrichment analysis was performed to determine its biological function. We preliminarily confirmed the role of via experiments and immunofluorescence staining.
This study found that SPAG6 expression was differentially expressed in cancers and at various tumor stages and grades. In stomach and esophageal carcinoma (STES), stomach adenocarcinoma (STAD), kidney renal clear cell carcinoma (KIRC), lung squamous cell carcinoma (LUSC), and adrenocortical carcinoma (ACC), SPAG6 expression was correlated with gender. SPAG6 expression was also found to be correlated with prognostic value, with low expression being associated with poor prognosis. Furthermore, SPAG6 expression was positively linked with immune-related cells in HNSC, chemokine receptors in LUSC, and immune checkpoint genes in THCA. Furthermore, SPAG6 overexpression suppressed the malignant phenotypes of THCA cells, manifested by slower proliferation and decreased migration. The different SPAG6 expression in THCA led to different malignant phenotypes, which are involved in the upregulation of DNA repair, MYC targets, peroxisome, and G2M checkpoint.
plays a significant role as an oncogene and can be used as a marker to predict the prognosis of cancer. influences both the tumor immune infiltration and microenvironment, making it a promising immunotherapeutic target for tumor therapy.
已有关于精子相关抗原6（SPAG6）在细胞骨架形成和生长锥稳定性方面作用的研究，但SPAG6如何影响肿瘤生长和发展尚不清楚。本研究旨在阐明SPAG6在泛癌中的作用，并通过实验验证了一些关于甲状腺癌（THCA）的发现。
我们利用从数据库收集的数据研究了SPAG6在泛癌中的作用。进一步分析评估其与预后、基因异质性、干性和肿瘤免疫的相关性。还鉴定了SPAG6的相互作用蛋白，并进行基因本体富集分析以确定其生物学功能。我们通过实验和免疫荧光染色初步证实了SPAG6的作用。
本研究发现SPAG6表达在癌症及不同肿瘤阶段和分级中存在差异。在胃癌和食管癌（STES）、胃腺癌（STAD）、肾透明细胞癌（KIRC）、肺鳞状细胞癌（LUSC）和肾上腺皮质癌（ACC）中，SPAG6表达与性别相关。还发现SPAG6表达与预后价值相关，低表达与预后不良相关。此外，SPAG6表达在头颈部鳞状细胞癌（HNSC）中与免疫相关细胞、在LUSC中与趋化因子受体以及在THCA中与免疫检查点基因呈正相关。此外，SPAG6过表达抑制了THCA细胞的恶性表型，表现为增殖减慢和迁移减少。THCA中不同的SPAG6表达导致不同的恶性表型，这涉及DNA修复、MYC靶点、过氧化物酶体和G2M检查点的上调。
SPAG6作为一种癌基因发挥重要作用，可作为预测癌症预后的标志物。SPAG6影响肿瘤免疫浸润和微环境，使其成为肿瘤治疗中一个有前景的免疫治疗靶点。
