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[使用膜过滤技术培养受氯损伤的大肠杆菌]

[Culturing of chlorine-damaged E. coli bacteria using the membrane filteration technic].

作者信息

Schubert R, Helm F

出版信息

Zentralbl Bakteriol Mikrobiol Hyg B. 1985 Jun;181(1-2):87-92.

PMID:3901607
Abstract

The number of chlorine-impaired E. coli organisms obtainable with the aid of the membrane-filtration-Endo-agar-method is so reduced that this method cannot be considered equivalent to liquid enrichment. Notwithstanding the well documented difficulties that arise from differences between batches of the same filters e. g. from problems to assure their standardization, the main obstacles do not stem from the questionable suitability of membrane filters but from the toxicity of the Endo-agar. The use of lactose-pepton (L.P.)-broth (German Standard Methods 1971) equally does not lead to satisfactory results if compared to the use of casein-soya (C.S.)-broth.

摘要

借助膜过滤-远藤琼脂法可获得的受氯损害的大肠杆菌数量减少到如此程度,以至于该方法不能被认为等同于液体富集法。尽管同一批次的过滤器之间存在差异会带来诸多有充分记录的困难,例如难以确保其标准化,但主要障碍并非源于膜过滤器的适用性存疑,而是源于远藤琼脂的毒性。与使用酪蛋白-大豆(C.S.)肉汤相比,使用乳糖-蛋白胨(L.P.)肉汤(德国标准方法,1971年)同样无法得到令人满意的结果。

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