School of Metallurgy and Materials Engineering, Iran University of Science and Technology, PO Box: 16846, Tehran, Iran.
Biomaterials Group, Department of Nanotechnology & Advanced Materials, Materials and Energy Research Center, PO Box: 31787-316, Karaj, Iran.
Biomed Mater. 2024 Jul 17;19(5). doi: 10.1088/1748-605X/ad606f.
The performance and long-term durability of dental implants hinge on the quality of bone integration and their resistance to bacteria. This research aims to introduce a surface modification strategy for zirconia implants utilizing femtosecond laser ablation techniques, exploring their impact on osteoblast cell behavior and bacterial performance, as well as the integral factors influencing the soft tissue quality surrounding dental implants. Ultrafast lasers were employed to craft nanoscale groove geometries on zirconia surfaces, with thorough analyses conducted using x-ray diffraction, scanning electron microscopy, atomic force microscopy, and water contact angle measurements. The study evaluated the response of human fetal osteoblastic cell lines to textured zirconia ceramics by assessing alkaline phosphatase activity, collagen I, and interleukin 1secretion over a 7 day period. Additionally, the antibacterial behavior of the textured surfaces was investigated using, a common culprit in infections associated with dental implants. Ciprofloxacin (CIP), a widely used antibacterial antibiotic, was loaded onto zirconia ceramic surfaces. The results of this study unveiled a substantial reduction in bacterial adhesion on textured zirconia surfaces. The fine biocompatibility of these surfaces was confirmed through the MTT assay and observations of cell morphology. Moreover, the human fetal osteoblastic cell line exhibited extensive spreading and secreted elevated levels of collagen I and interleukin 1in the modified samples. Drug release evaluations demonstrated sustained CIP release through a diffusion mechanism, showcasing excellent antibacterial activity against pathogenic bacteria, including, and.
种植体的性能和长期耐用性取决于骨整合的质量及其对细菌的抵抗力。本研究旨在介绍一种利用飞秒激光烧蚀技术对氧化锆种植体进行表面改性的策略，探讨其对成骨细胞行为和细菌性能的影响，以及影响周围软组织质量的整体因素。超快激光器在氧化锆表面上制造纳米级槽状结构，通过 X 射线衍射、扫描电子显微镜、原子力显微镜和水接触角测量对其进行了全面分析。通过评估碱性磷酸酶活性、胶原 I 和白细胞介素 1 的分泌，研究了人胎成骨细胞系对纹理化氧化锆陶瓷的反应，为期 7 天。此外，还使用常见的与牙种植体感染相关的细菌研究了纹理表面的抗菌行为。将广泛用于治疗感染的抗生素环丙沙星 (CIP) 负载到氧化锆陶瓷表面。研究结果表明，纹理化氧化锆表面上的细菌黏附显著减少。MTT 测定和细胞形态观察证实了这些表面的良好生物相容性。此外，人胎成骨细胞系在改性样本中表现出广泛的扩散，并分泌高水平的胶原 I 和白细胞介素 1。药物释放评估表明，通过扩散机制实现了 CIP 的持续释放，对包括 和 在内的致病菌表现出了优异的抗菌活性。
