Department of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, Poznań, Poland.
Postepy Biochem. 2024 May 23;70(1):52-56. doi: 10.18388/pb.2021_509.
Environmental carcinogens exert their carcinogenic effects by forming DNA adducts. This type of DNA damage can also be formed endogenously as a result of, e.g., oxidative damage. Unrepaired DNA adducts may induce mutations in critical genes, leading to the initiation of chemical carcinogenesis. Therefore, detection, identification, and quantification of DNA adducts is essential for cancer risk assessment. Over the last 50 years, the major DNA adducts formed by different classes of environmental carcinogens were characterized. With the development of techniques such as 32P-postlabeling, their measurement was implemented into molecular epidemiology. Advances in liquid chromatography-tandem mass spectrometry (LC-MS ) made the measurement of adducts more precise and allowed to gain knowledge about their identity and structures. Therefore, opened the way to DNA adductomics, the "omics" approach investigating DNA adducts comprehensively, similarly to proteomics. This review presents the historical perspective of DNA adducts research and the emerging field of adductomics.
环境致癌物通过形成 DNA 加合物发挥其致癌作用。这种类型的 DNA 损伤也可能是内源性的，例如氧化损伤的结果。未修复的 DNA 加合物可能会在关键基因中诱导突变，从而引发化学致癌作用。因此，DNA 加合物的检测、鉴定和定量对于癌症风险评估至关重要。在过去的 50 年中，已经对不同类别的环境致癌物形成的主要 DNA 加合物进行了表征。随着 32P-后标记等技术的发展，其测量已被纳入分子流行病学。液相色谱-串联质谱（LC-MS）的进步使加合物的测量更加精确，并使我们能够了解它们的身份和结构。因此，开辟了 DNA 加合物组学的道路，这是一种类似于蛋白质组学的全面研究 DNA 加合物的“组学”方法。本综述介绍了 DNA 加合物研究的历史背景和新兴的加合物组学领域。
