Universidade Federal de Mato Grosso, Instituto de Ciências Biológicas e da Saúde, Laboratório de Fisiologia de Sistemas e Toxicologia Reprodutiva, Av. Valdon Varjão, 6390, 78605-091 Barra do Garças, MT, Brazil.
Programa de Pós-Graduação em Tocoginecologia, Universidade Estadual Paulista, Faculdade de Medicina, Laboratório de Pesquisa Experimental em Ginecologia e Obstetrícia, Av. Prof. Mário Rubens Guimarães Montenegro, s/n, 18618-687 Botucatu, SP, Brazil.
An Acad Bras Cienc. 2024 Jul 15;96(3):e20230604. doi: 10.1590/0001-3765202420230604. eCollection 2024.
This study aims to evaluate the phytochemical properties of Bauhinia holophylla (Bong.) Steud leaf extract, and their impact on maternal reproductive and fetal development in diabetic rats. For this, adult female Wistar rats (100 days of life) received streptozotocin (40 mg/Kg, intraperitoneal) for induction of diabetes, were mated and distributed into four groups: Nondiabetic; Nondiabetic given B. holophylla; Diabetic; and Diabetic given B. holophylla. The plant extract was given by gavage at increasing doses: 200, 400, and 800 mg/Kg. At day 21 of pregnancy, liver and blood samples were obtained for oxidative parameters and biochemical analysis, respectively. The uterus was removed for maternal-fetal outcomes. Phytochemical analysis showed a high content of phenolic components and biogenic amines. B. holophylla extract did not alter the glycemic levels but improved the lipid profile in diabetic animals. Besides that, the number of live fetuses and maternal weight gain were decreased in Diabetic group, and were not observed in animals treated. The group Diabetic treated presented a higher percentage of fetuses classified as adequate for gestational age compared to the Diabetic group. However, the treatment with plant extract caused embryo losses, fetal growth restriction, and teratogenicity in nondiabetic rats. Thus, the indiscriminate consumption requires carefulness.
本研究旨在评估羊蹄甲（Bauhinia holophylla（Bong.）Steud）叶提取物的植物化学成分特性及其对糖尿病大鼠母体生殖和胎儿发育的影响。为此，成年雌性 Wistar 大鼠（100 天龄）接受链脲佐菌素（40mg/Kg，腹腔内注射）诱导糖尿病，然后交配并分为四组：非糖尿病组；非糖尿病给予羊蹄甲；糖尿病组；和糖尿病给予羊蹄甲。通过灌胃给予植物提取物，剂量逐渐增加：200、400 和 800mg/Kg。在妊娠第 21 天，采集肝脏和血液样本分别用于氧化参数和生化分析。取出子宫以评估母婴结局。植物化学成分分析显示酚类成分和生物胺含量高。羊蹄甲提取物并未改变血糖水平，但改善了糖尿病动物的血脂谱。此外，糖尿病组的活胎数和母体体重增加减少，而在接受治疗的动物中则没有观察到。与糖尿病组相比，接受植物提取物治疗的糖尿病组中胎儿达到适当胎龄的比例更高。然而，植物提取物的治疗导致了非糖尿病大鼠的胚胎丢失、胎儿生长受限和致畸性。因此，这种植物提取物的滥用需要引起警惕。
