Ni Jiancong, Yang Bifang, Liu Liyang, Dai Xiaohui, Yang Weiqiang, Wang Qingxiang, Chen Xiaoping, Song Zhiping, Lin Zhenyu
Fujian Provincial Key Laboratory of Modern Analytical Science and Separation Technology, Fujian Provincial Key Laboratory of Pollution Monitoring and Control, College of Chemistry, Chemical Engineering and Environment, Minnan Normal University, Zhangzhou 363000, China.
Zhangzhou Product Quality Inspection Institute, Zhangzhou 363000, Fujian, China.
Anal Chem. 2024 Jul 17. doi: 10.1021/acs.analchem.4c02789.
Electrochemiluminescence (ECL) sensors have been widely developed because of their high sensitivity and low background. However, most of them suffered from tedious probe modification on the electrode and cross-interferences within the sensing and reporting reactions. The bipolar electrode based ECL (BPE-ECL) can effectively eliminate interference by physically separating the sensing and reporting cells, but there is still a need for exogenous electroactive indicators to transduce the variations between two poles of a BPE. Herein, based on the discovery that conductivity can be regulated in aqueous medium by homogeneous bioreaction, we showed a novel BPE-ECL sensing platform that combined the conductivity-based biosensing technology with ECL reporting system for the first time. Compared to many short nucleic acids, the target induced a hybridization chain reaction to produce the long nucleic acid aggregates, resulting in a conductivity decrease of the sensing cell and finally reducing the ECL response in the reporting cell. The BPE-ECL platform has already been applied to detect microRNA-21 for a demonstration. This innovative system not only separates the target sensing and reporting reactions but also avoids the use of electrochemical indicators for measurement. The BPE-ECL biosensing platform can be developed to detect different targets by changing the probe used.
由于具有高灵敏度和低背景，电化学发光（ECL）传感器得到了广泛发展。然而，它们中的大多数存在电极上探针修饰繁琐以及传感和报告反应中的交叉干扰问题。基于双极电极的ECL（BPE-ECL）可以通过物理分离传感和报告单元来有效消除干扰，但仍需要外源性电活性指示剂来传导BPE两极之间的变化。在此，基于在水性介质中通过均相生物反应可调节电导率这一发现，我们首次展示了一种将基于电导率的生物传感技术与ECL报告系统相结合的新型BPE-ECL传感平台。与许多短核酸相比，靶标诱导杂交链式反应产生长核酸聚集体，导致传感单元的电导率降低，最终降低报告单元中的ECL响应。BPE-ECL平台已被应用于检测微小RNA-21以作演示。这种创新系统不仅分离了靶标传感和报告反应，还避免了使用电化学指示剂进行测量。通过改变所使用的探针，BPE-ECL生物传感平台可以被开发用于检测不同的靶标。
