Ge Junjun, Yin Tengyue, Zhang Haoyang, Cao Yue, Liu Juan, Zhu Jun-Jie, Zhou Yang, Wang Yuanyuan
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University Nanjing 210093 China
Key Laboratory for Organic Electronics & Information Displays, Institute of Advanced Materials, Nanjing University of Posts & Telecommunications (NJUPT) Nanjing 210023 PR China
Chem Sci. 2025 Jan 22;16(8):3671-3679. doi: 10.1039/d4sc07800d. eCollection 2025 Feb 19.
Semiconductor magic-sized nanoclusters (MSCs) possess atomic-level compositional precision and ultrasmall dimensions, allowing accurate modulation of electrochemiluminescence (ECL) properties, essential for advanced bioanalytical applications. However, low intrinsic ECL intensity and poor stability in bipolar electrode (BPE)-ECL systems hinder their broader use. In this work, we addressed these limitations through doping and direct optical crosslinking strategies, achieving a 24-fold boost in the ECL signal and a fivefold stability increase for doped (CdS):Ag MSCs compared with original (CdS) MSCs. The resulting BPE-ECL biosensing platform was used for the sensitive detection of glucose with a linear detection range of 10 μM to 1 mM and a detection limit of 3.64 μM. This approach provides a robust strategy to enhance MSC-based ECL biosensing, paving the way for ultrasensitive, stable biosensors for clinical diagnostics and bioanalysis.
半导体魔法尺寸纳米团簇（MSCs）具有原子级的组成精度和超小尺寸，能够精确调制电化学发光（ECL）特性，这对于先进的生物分析应用至关重要。然而，双极电极（BPE）-ECL系统中固有的低ECL强度和较差的稳定性阻碍了它们的更广泛应用。在这项工作中，我们通过掺杂和直接光交联策略解决了这些限制，与原始的（CdS）MSCs相比，掺杂的（CdS）:Ag MSCs的ECL信号提高了24倍，稳定性提高了五倍。由此产生的BPE-ECL生物传感平台用于葡萄糖的灵敏检测，线性检测范围为10 μM至1 mM，检测限为3.64 μM。这种方法为增强基于MSCs的ECL生物传感提供了一种强大的策略，为用于临床诊断和生物分析的超灵敏、稳定生物传感器铺平了道路。
