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用电泳技术在类蠕虫胶束网络中捕获病毒基因组 使用肽核酸两亲体和双链 DNA 寡聚物

Electrophoretically Snagging Viral Genomes in Wormlike Micelle Networks Using Peptide Nucleic Acid Amphiphiles and dsDNA Oligomers.

机构信息

Department of Chemical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, United States.

出版信息

Biomacromolecules. 2024 Aug 12;25(8):4891-4897. doi: 10.1021/acs.biomac.4c00332. Epub 2024 Jul 17.

DOI:10.1021/acs.biomac.4c00332
PMID:39017713
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11322999/
Abstract

We demonstrate that the attachment of 30-170 bp dsDNA oligomers to ssDNA viral genomes gives a significant additional mobility shift in micelle-tagging electrophoresis (MTE). In MTE, a modified peptide nucleic acid amphiphile is attached to the viral genome to bind drag-inducing micelles present in capillary electrophoresis running buffers. Further attachment of 30-170 bp dsDNA oligomers drastically shifts the mobility of the 5.1 kB ssDNA genome of mouse minute virus (MMV), providing a new mechanism to improve resolution in CE-based analysis of kilobase nucleic acids. A model based on biased-reptation electrophoresis, end-labeled free-solution electrophoresis, and Ferguson gel-filtration theory is presented to describe the observed mobility shifts.

摘要

我们证明，将 30-170bp 的双链 DNA 寡核苷酸与单链 DNA 病毒基因组结合，在胶束标记电泳 (MTE) 中会产生显著的额外迁移率变化。在 MTE 中，一种修饰的肽核酸两亲分子与病毒基因组结合，以结合毛细管电泳运行缓冲液中存在的诱导拖曳的胶束。进一步结合 30-170bp 的双链 DNA 寡核苷酸会极大地改变 5.1kb 的单链 DNA 基因组的迁移率，为基于 CE 的千碱基核酸分析中提高分辨率提供了一种新机制。提出了一个基于有偏的缠结电泳、末端标记的自由溶液电泳和 Ferguson 凝胶过滤理论的模型，以描述观察到的迁移率变化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be01/11322999/9ee1751b511a/bm4c00332_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be01/11322999/6c62b5b5891b/bm4c00332_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be01/11322999/8b6043c80946/bm4c00332_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be01/11322999/f4c165be5944/bm4c00332_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be01/11322999/b44fc8273bf2/bm4c00332_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be01/11322999/9ee1751b511a/bm4c00332_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be01/11322999/6c62b5b5891b/bm4c00332_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be01/11322999/8b6043c80946/bm4c00332_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be01/11322999/f4c165be5944/bm4c00332_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be01/11322999/b44fc8273bf2/bm4c00332_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be01/11322999/9ee1751b511a/bm4c00332_0005.jpg

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本文引用的文献

1
Quantification of mRNA in Lipid Nanoparticles Using Mass Spectrometry.使用质谱法对脂质纳米颗粒中的 mRNA 进行定量。
Anal Chem. 2024 Jan 23;96(3):1214-1222. doi: 10.1021/acs.analchem.3c04406. Epub 2024 Jan 8.
2
Rapid, multiplexed detection of the let-7 miRNA family using γPNA amphiphiles in micelle-tagging electrophoresis.利用 γPNA 两亲体在胶束标记电泳中快速、多重检测 let-7 miRNA 家族。
Biopolymers. 2022 Feb;113(2):e23479. doi: 10.1002/bip.23479. Epub 2021 Oct 13.
3
Analytical methods to characterize recombinant adeno-associated virus vectors and the benefit of standardization and reference materials.
分析方法用于鉴定重组腺相关病毒载体，标准化和参考物质的优势。
Curr Opin Biotechnol. 2021 Oct;71:65-76. doi: 10.1016/j.copbio.2021.06.025. Epub 2021 Jul 15.
4
Electrophoretic Mobility of DNA in Solutions of High Ionic Strength.高离子强度溶液中 DNA 的电泳迁移率。
Biophys J. 2020 Jun 2;118(11):2783-2789. doi: 10.1016/j.bpj.2020.02.034. Epub 2020 Apr 30.
5
Viral contamination in biologic manufacture and implications for emerging therapies.生物制品制造中的病毒污染及其对新兴疗法的影响。
Nat Biotechnol. 2020 May;38(5):563-572. doi: 10.1038/s41587-020-0507-2. Epub 2020 Apr 27.
6
A 502-Base Free-Solution Electrophoretic DNA Sequencing Method Using End-Attached Wormlike Micelles.一种基于 502 碱基无胶电泳 DNA 测序方法，使用端接的蠕虫状胶束。
Anal Chem. 2015 Nov 17;87(22):11433-40. doi: 10.1021/acs.analchem.5b02931. Epub 2015 Nov 2.
7
High affinity γPNA sandwich hybridization assay for rapid detection of short nucleic acid targets with single mismatch discrimination.高亲和力 γPNA 夹心杂交分析快速检测具有单碱基错配区分能力的短核酸靶标。
Biomacromolecules. 2013 Jul 8;14(7):2253-61. doi: 10.1021/bm400388a. Epub 2013 Jun 18.
8
Beyond gel electrophoresis: microfluidic separations, fluorescence burst analysis, and DNA stretching.超越凝胶电泳：微流控分离、荧光猝发分析和DNA拉伸。
Chem Rev. 2013 Apr 10;113(4):2584-667. doi: 10.1021/cr3002142. Epub 2012 Nov 12.
9
Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction.Primer-BLAST：一种用于设计聚合酶链反应（PCR）目标特异性引物的工具。
BMC Bioinformatics. 2012 Jun 18;13:134. doi: 10.1186/1471-2105-13-134.
10
Sequence-unrestricted, Watson-Crick recognition of double helical B-DNA by (R)-miniPEG-γPNAs.（R）- 小聚乙二醇 -γPNAs 对双链 B-DNA 的无序列限制、沃森 - 克里克识别。
Chembiochem. 2012 Jan 2;13(1):56-60. doi: 10.1002/cbic.201100646. Epub 2011 Dec 1.