Todorov Todor I, Morris Michael D
Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA.
Electrophoresis. 2002 Apr;23(7-8):1033-44. doi: 10.1002/1522-2683(200204)23:7/8<1033::AID-ELPS1033>3.0.CO;2-7.
We present a study of the separation of RNA, single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) in semidilute linear hydroxyethylcellulose (HEC) solution. Our results strive to provide a better understanding of the mechanisms of nucleic acid migration during electrophoresis in polymer solutions under native and denaturing conditions. From a study of the dependence of mobility on chain length and applied electric field, we found that RNA and ssDNA show better separation and higher resolution over a larger range of sizes compared to dsDNA. In addition, RNA reptation without orientation extends to longer chain lengths in comparison to ssDNA, possibly as a result of different type of short-lived secondary structure formations. Such a comparative study between nucleic acid capillary electrophoresis helps to optimize RNA separation and provides better understanding of RNA migration mechanisms in semidilute polymer solutions under denaturing conditions.
我们展示了一项在半稀释线性羟乙基纤维素（HEC）溶液中分离RNA、单链DNA（ssDNA）和双链DNA（dsDNA）的研究。我们的结果致力于更好地理解在天然和变性条件下，核酸在聚合物溶液中电泳时的迁移机制。通过研究迁移率对链长和外加电场的依赖性，我们发现与dsDNA相比，RNA和ssDNA在更大的尺寸范围内表现出更好的分离效果和更高的分辨率。此外，与ssDNA相比，无取向的RNA链的爬行延伸到更长的链长，这可能是由于不同类型的短寿命二级结构形成的结果。这种核酸毛细管电泳之间的比较研究有助于优化RNA分离，并更好地理解变性条件下半稀释聚合物溶液中RNA的迁移机制。
