通过 CUT&Tag 对 B 细胞亚群进行表观基因组分析。
Epigenomic Profiling of B Cell Subsets by CUT&Tag.
机构信息
Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, USA.
出版信息
Methods Mol Biol. 2024;2826:65-77. doi: 10.1007/978-1-0716-3950-4_6.
Abstract
Epigenetic programs play a key role in regulating the development and function of immune cells. However, conventional methods for profiling epigenetic mechanisms, such as the post-translational modifications to histones, present several technical challenges that prevent a complete understanding of gene regulation. Here, we provide a detailed protocol of the Cleavage Under Targets and Tagmentation (CUT&Tag) chromatin profiling technique for identifying histone modifications in human and mouse lymphocytes.
摘要
表观遗传程序在调节免疫细胞的发育和功能方面发挥着关键作用。然而，传统的表观遗传机制分析方法，如组蛋白的翻译后修饰，存在一些技术挑战，阻碍了对基因调控的全面理解。在这里，我们提供了一种详细的靶向切割和标签（CUT&Tag）染色质分析技术的方案，用于鉴定人类和小鼠淋巴细胞中的组蛋白修饰。
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