评估人血浆细胞的体外存活情况。

Assessing the Survival of Human Plasma Cells Ex Vivo.

机构信息

Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC, Australia.

Immunity Program, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.

出版信息

Methods Mol Biol. 2024;2826:219-230. doi: 10.1007/978-1-0716-3950-4_16.

DOI:10.1007/978-1-0716-3950-4_16

PMID:39017896

Abstract

One way memory B cells provide protection is by rapidly differentiating into plasma cells. Plasma cells are vital in providing long-term protection against pathogens; however, they can also be detrimental to health in the case of antibody-mediated autoimmunity. Therefore, compounds which modulate the survival of plasma cells have been of interest for therapeutic intervention. Investigation of ex vivo plasma cell survival has previously been limited by the low frequency of plasma cells in the blood. Here we describe a novel ex vivo culture system that only requires 3000-5000 cells per condition. This method permits the assessment of human plasma cell survival derived from blood and can assess the impact of small molecule inhibitors on plasma cell viability.

摘要

记忆 B 细胞提供保护的一种方式是快速分化为浆细胞。浆细胞在提供针对病原体的长期保护方面至关重要；然而，在抗体介导的自身免疫的情况下，它们也可能对健康有害。因此，调节浆细胞存活的化合物一直是治疗干预的关注点。体外浆细胞存活的研究以前受到血液中浆细胞频率低的限制。在这里，我们描述了一种新的体外培养系统，每个条件只需要 3000-5000 个细胞。该方法允许评估来自血液的人浆细胞存活，并可以评估小分子抑制剂对浆细胞活力的影响。