Division of Neuroscience, Oregon National Primate Research Center, Oregon Health & Science University, 505 NW 185th Avenue, Beaverton, OR, 97006, USA.
Najít Technologies, Inc, 505 NW 185th Avenue, Beaverton, OR, 97006, USA.
Nat Commun. 2017 Nov 24;8(1):1781. doi: 10.1038/s41467-017-01901-w.
Pre-existing serum antibodies play an important role in vaccine-mediated protection against infection but the underlying mechanisms of immune memory are unclear. Clinical studies indicate that antigen-specific antibody responses can be maintained for many years, leading to theories that reactivation/differentiation of memory B cells into plasma cells is required to sustain long-term antibody production. Here, we present a decade-long study in which we demonstrate site-specific survival of bone marrow-derived plasma cells and durable antibody responses to multiple virus and vaccine antigens in rhesus macaques for years after sustained memory B cell depletion. Moreover, BrdU cells with plasma cell morphology can be detected for 10 years after vaccination/BrdU administration, indicating that plasma cells may persist for a prolonged period of time in the absence of cell division. On the basis of these results, long-lived plasma cells represent a key cell population responsible for long-term antibody production and serological memory.
预先存在的血清抗体在疫苗介导的抗感染保护中发挥着重要作用，但免疫记忆的潜在机制尚不清楚。临床研究表明，抗原特异性抗体反应可以维持多年，这导致了这样的理论，即记忆 B 细胞的再激活/分化为浆细胞是维持长期抗体产生所必需的。在这里，我们提出了一项长达十年的研究，该研究表明，在恒河猴中，持续耗尽记忆 B 细胞后，骨髓来源的浆细胞可以在特定部位存活，并且对多种病毒和疫苗抗原产生持久的抗体反应多年。此外，接种疫苗/给予 BrdU 后 10 年内可检测到具有浆细胞形态的 BrdU 细胞，表明在没有细胞分裂的情况下，浆细胞可能会持续存在较长时间。基于这些结果，长寿浆细胞代表了负责长期抗体产生和血清记忆的关键细胞群体。
