Sun Tong, Wu Lei, Cui Mengyuan, Zhao Guisen
Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China.
Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing, 210009, China.
J Fluoresc. 2024 Jul 17. doi: 10.1007/s10895-024-03778-7.
Nitroreductase (NTR) is to be pivotal in the biodegradation of nitroaromatics. NTR is produced in tumor tissues under hypoxic conditions, which is one of the markers for early tumor diagnosis. In this study, a novel probe FD-NTR was developed for NTR detection. Probe FD-NTR can exhibit a specific reaction with NTR in the presence of NADH. The probe displayed satisfactory selectivity and sensitivity towards NTR with a calculated detection limit of 12 ng/mL. Under the conditions of low cytotoxic hypoxia, the FD-NTR probe has shown successful application in imaging both MCF-7 cells and tumor tissues, which indicated that the FD-NTR probe holds promising application prospects for detecting NTR in tumors.
硝基还原酶（NTR）在硝基芳烃的生物降解中起关键作用。NTR在缺氧条件下于肿瘤组织中产生，这是早期肿瘤诊断的标志物之一。在本研究中，开发了一种用于检测NTR的新型探针FD-NTR。探针FD-NTR在烟酰胺腺嘌呤二核苷酸（NADH）存在下可与NTR发生特异性反应。该探针对NTR表现出令人满意的选择性和灵敏度，计算得出的检测限为12 ng/mL。在低细胞毒性缺氧条件下，FD-NTR探针已成功应用于MCF-7细胞和肿瘤组织的成像，这表明FD-NTR探针在检测肿瘤中的NTR方面具有广阔的应用前景。
