Matsuda Y, Takada A, Sato H, Yasuhara M, Takase S
Alcohol Clin Exp Res. 1985 Jul-Aug;9(4):366-70. doi: 10.1111/j.1530-0277.1985.tb05561.x.
To establish clearly what the pathogenetic differences are in the hepatocytic ballooning between human alcoholic and nonalcoholic liver diseases, hepatic microtubules were examined by morphometric and biochemical methods, and staining of transferrin was carried out on liver sections immunohistochemically. Microheterogeneity of serum transferrin was also detected by immunofixation after isoelectric focusing. Hepatic microtubules were significantly decreased in alcoholic liver disease, and transferrin was clearly stained in the ballooned hepatocytes of alcoholic liver disease but not in nonalcoholic liver disease. The degree to which transferrin was stained was related to hepatic microtubular contents and also related to the appearance of the microheterogeneity of serum transferrin in alcoholic liver disease. These findings indicate that ballooning of hepatocytes in alcoholic liver disease, but not in nonalcoholic liver disease, is caused by the accumulation of exportable proteins due to impairment of microtubular polymerization. This accumulation might be related to the inhibition of glycosylation and secretion of glycoproteins by the impairment of microtubular functions. From these results, it was determined that staining of transferrin in the liver may be useful for differentiation of the etiology of liver diseases.
为了明确人类酒精性肝病和非酒精性肝病中肝细胞气球样变的发病机制差异，采用形态计量学和生化方法检测肝微管，并在肝切片上进行转铁蛋白的免疫组织化学染色。等电聚焦后通过免疫固定法也检测了血清转铁蛋白的微异质性。酒精性肝病中肝微管显著减少，转铁蛋白在酒精性肝病的气球样变肝细胞中明显染色，而在非酒精性肝病中则未染色。转铁蛋白的染色程度与肝微管含量有关，也与酒精性肝病中血清转铁蛋白微异质性的出现有关。这些发现表明，酒精性肝病而非非酒精性肝病中的肝细胞气球样变是由于微管聚合受损导致可输出蛋白积累所致。这种积累可能与微管功能受损导致糖蛋白糖基化和分泌受抑制有关。根据这些结果，确定肝脏中转铁蛋白的染色可能有助于区分肝病的病因。
