Institute of Sciences and Technologies for Sustainable Energy and Mobility (CNR-STEMS), Via G. Marconi, 4, Naples 80125, Italy.
Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via Pansini, 5, Naples 80131, Italy.
ACS Appl Mater Interfaces. 2024 Jul 31;16(30):38956-38967. doi: 10.1021/acsami.4c06000. Epub 2024 Jul 17.
This work reports the production of biocompatible thin layers for biomedical applications based on a graphene-like material (GL), a graphene-related material (GRM) obtained from carbon black. GL was combined in a hybrid fashion with polydopamine (pDA), a mussel-inspired water-resistant wet adhesive bonding obtained by the oxidative polymerization of dopamine (DA), and polyvinyl pyrrolidinone (PVP), a nontoxic synthetic polymer with intrinsic adhesion properties, to obtain a tighter adhesion of the thin layer to the substrate (silicone slices). Matrix-assisted pulsed laser evaporation (MAPLE) was used to coat PDMS slices with thin films of GL-pDA and GL-PVP directly from their frozen suspensions in water. The results indicate that the relevant chemical-physical characteristics of both thin films (evidenced by FTIR and AFM) were maintained after MAPLE deposition and that the films exhibit uniformity also at the nanometric level. After deposition, the GL-pDA and GL-PVP films underwent a biological survey toward murine fibroblasts (NIH3T3), human keratinocytes (HaCAT), and human cervical adenocarcinoma epithelial-like (HeLa) cells to assess the feasibility of this approach. Results indicate that both the GL-pDA and GL-PVP films did not perturb the biological parameters evaluated, including cytoskeleton alterations. Both hybrid films enhanced the effects of GL on cellular vitality across all cell lines. Specifically, the GL-pDA film exhibited a more stable effect over time (up to 72 h), whereas the GL-PVP film behaved similarly to the GL film in NIH3T3 and HeLa cell lines after long-term exposure. These promising results make the GL-pDA and GL-PVP films potential candidates for the manufacture of coated flexible devices for biomedical applications.
这项工作报道了基于类石墨烯材料（GL）的生物相容性薄膜的制备，GL 是从炭黑中获得的一种与石墨烯相关的材料（GRM）。GL 以混合方式与聚多巴胺（pDA）结合，pDA 是一种通过多巴胺（DA）的氧化聚合获得的、具有耐水粘性的贻贝启发型水不溶性湿胶黏剂，同时还与聚乙烯基吡咯烷酮（PVP）结合，PVP 是一种具有内在黏附特性的无毒合成聚合物，以获得薄膜与基底（硅树脂片）更紧密的黏附。基质辅助脉冲激光蒸发（MAPLE）用于直接从其在水中的冷冻悬浮液中在 PDMS 片上涂覆 GL-pDA 和 GL-PVP 的薄膜。结果表明，两种薄膜的相关理化特性（通过 FTIR 和 AFM 证明）在 MAPLE 沉积后得以保持，并且在纳米级水平上也具有均匀性。沉积后，GL-pDA 和 GL-PVP 薄膜对鼠成纤维细胞（NIH3T3）、人角质形成细胞（HaCAT）和人宫颈腺癌上皮样（HeLa）细胞进行了生物学调查，以评估这种方法的可行性。结果表明，GL-pDA 和 GL-PVP 薄膜均未干扰所评估的生物学参数，包括细胞骨架改变。两种混合薄膜均增强了 GL 对所有细胞系细胞活力的影响。具体而言，GL-pDA 薄膜在较长时间内（长达 72 小时）表现出更稳定的效果，而 GL-PVP 薄膜在长期暴露后在 NIH3T3 和 HeLa 细胞系中的行为类似于 GL 薄膜。这些有前景的结果使 GL-pDA 和 GL-PVP 薄膜成为用于制造用于生物医学应用的涂覆柔性器件的潜在候选物。
