Alfe Michela, Minopoli Giuseppina, Tartaglia Massimiliano, Gargiulo Valentina, Caruso Ugo, Pepe Giovanni Piero, Ausanio Giovanni
Institute of Sciences and Technologies for Sustainable Energy and Mobility (CNR-STEMS), P.le V. Tecchio 80, 80125 Naples, Italy.
Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, via Pansini, 5, 80131 Naples, Italy.
Nanomaterials (Basel). 2022 Oct 18;12(20):3663. doi: 10.3390/nano12203663.
In this study, matrix-assisted pulsed laser evaporation (MAPLE) was used to deposit graphene-like materials (GL), a new class of biocompatible graphene-related materials (GRMs) obtained from a controlled top-down demolition of a carbon black, on silicone slices to test their potential use as functional coating on invasive medical devices as indwelling urinary catheters. Results indicate that the relevant chemical-physical features of the deposit (controlled by FTIR and AFM) were maintained after MAPLE deposition. After deposition, GL films underwent a biological survey toward target cellular lines (murine fibroblast NIH3T3, human keratinocytes HaCAT and the human cervical adenocarcinoma epithelial-like HeLa). Results indicate that the GL films did not lead to any perturbations in the different biological parameters evaluated. The presented results and the possibility to further functionalize the GL or combine them with other functional materials in a hybrid fashion to assure a tighter adhesion onto the substrate for use in harsh conditions open the door to practical applications of these new-concept medical devices (drug delivery, next generation flexible devices, multifunctional coatings) paving the way to the prevention of nosocomial infections driven by catheterization through antibiotics-free approaches.
在本研究中，采用基质辅助脉冲激光蒸发（MAPLE）法将类石墨烯材料（GL）沉积在硅片上，该材料是通过对炭黑进行可控的自上而下分解而获得的一类新型生物相容性石墨烯相关材料（GRM），以测试其作为留置尿管等侵入性医疗器械功能涂层的潜在用途。结果表明，在MAPLE沉积后，沉积物的相关化学物理特性（由傅里叶变换红外光谱（FTIR）和原子力显微镜（AFM）控制）得以保持。沉积后，对GL薄膜针对目标细胞系（小鼠成纤维细胞NIH3T3、人角质形成细胞HaCAT和人宫颈腺癌上皮样HeLa）进行了生物学检测。结果表明，GL薄膜在评估的不同生物学参数中未导致任何干扰。所呈现的结果以及进一步对GL进行功能化或与其他功能材料以混合方式结合以确保在恶劣条件下更紧密地粘附在基材上的可能性，为这些新概念医疗器械（药物递送、下一代柔性设备、多功能涂层）的实际应用打开了大门，为通过无抗生素方法预防导尿管相关医院感染铺平了道路。
