Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, 100084 Beijing, PR China; Beijing Laboratory for Environmental Frontier Technologies, 100084 Beijing, PR China.
Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, 100084 Beijing, PR China; Beijing Laboratory for Environmental Frontier Technologies, 100084 Beijing, PR China.
J Hazard Mater. 2024 Sep 5;476:135136. doi: 10.1016/j.jhazmat.2024.135136. Epub 2024 Jul 8.
This study investigates the effects of chlorine dioxide (ClO) disinfection on the community structure, regrowth potential, and metabolic product secretion of disinfection-residual bacteria (DRB) in secondary effluent (SE), denitrification filter effluent (DFE), and ultrafiltration effluent (UE). Results show that ClO effectively reduces bacteria in SE and UE, achieving log removal values exceeding 3 at 1 mg/L within 30 min. A salient positive correlation (R > 0.95) exists between changes in total fluorescence intensity and disinfection efficacy. Post-treatment, Acinetobacter abundance increased in SE, while Pseudomonas decreased in DFE and UE. At lower ClO concentrations, Staphylococcus, Mycobacterium, Aeromonas, and Lactobacillus increased in DFE, but decreased at higher concentrations. After storage, bacterial counts in disinfected samples exceeded those in the control group, surpassing 10 CFU/mL. Despite an initial decline, species richness and evenness partially recovered but remained lower than control levels. Culturing DRB for 72 h showed elevated extracellular polymeric substances (EPS) secretion, quantified as total organic carbon (TOC), ranging from 5 to 27 mg/L, with significantly higher EPS in the disinfection group. Parallel factor analysis with self-organizing maps (PARAFAC-SOM) effectively differentiated water sample types and EPS fluorescent substances, underscoring the potential of three-dimensional fluorescence as an indirect measure of ClO disinfection efficacy.
本研究考察了二氧化氯（ClO）消毒对二级出水（SE）、反硝化滤池出水（DFE）和超滤出水（UE）中消毒残留细菌（DRB）的群落结构、再生长潜力和代谢产物分泌的影响。结果表明，ClO 能有效降低 SE 和 UE 中的细菌，在 1mg/L 下 30min 内对数去除值超过 3。总荧光强度的变化与消毒效果之间存在显著的正相关（R>0.95）。处理后，SE 中的不动杆菌丰度增加，而 DFE 和 UE 中的假单胞菌减少。在较低的 ClO 浓度下，DFE 中的葡萄球菌、分枝杆菌、气单胞菌和乳杆菌增加，但在较高浓度下减少。储存后，消毒样品中的细菌计数超过对照组，超过 10CFU/mL。尽管最初有所下降，但物种丰富度和均匀度部分恢复，但仍低于对照组水平。培养 72h 的 DRB 表现出更高的胞外聚合物物质（EPS）分泌，用总有机碳（TOC）定量，范围从 5 到 27mg/L，消毒组的 EPS 明显更高。平行因子分析与自组织映射（PARAFAC-SOM）有效地将水样类型和 EPS 荧光物质区分开来，突出了三维荧光作为 ClO 消毒效果间接测量的潜力。
