Cheng Chun-Yan, Li Hai-Bei, Liang Yong-Bing, Shi Dan-Yang, Chen Zheng-Shan, Yang Dong, Jiao Qiao-Rui, Shao Yi-Fan, Li Jun-Wen, Jin Min
Tianjin Key Laboratory of Risk Assessment and Control for Environment & Food Safety, Tianjin Institute of Environmental Medicine and Operational Medicine, Tianjin 300050, China.
Huan Jing Ke Xue. 2021 Apr 8;42(4):1939-1945. doi: 10.13227/j.hjkx.202008182.
In order to study the effects of chlorine dioxide (ClO) disinfection on the super antibiotic resistance genes (SARGs), the final effluents before and after chlorine dioxide were sampled throughout one year in a wastewater treatment plant (WWTP). The bacteria and extracellular nucleic acid were collected using microporous membrane filtration and nucleic acid adsorption particles, respectively. A total of 9 SARGs was detected through a quantitative real-time polymerase chain reaction (qPCR). The results revealed that both intracellular and extracellular , , and could be positively detected in the samples. Overall, ClO disinfection enhanced the relative abundance of the iSARGs (<0.05), exhibiting a seasonal pattern, and increasing in the spring, summer, and autumn. In spring, it improved the most, up to twice the abundance. No SARGs were detected positive in the winter, either intracellularly or extracellularly. There was no significant variation in the concentrations of eSARGs before and after ClO disinfection. Therefore, ClO disinfection cannot effectively remove iSARGs and eSARGs in the final effluent from the WWTP.
为研究二氧化氯（ClO）消毒对超级抗生素抗性基因（SARGs）的影响，在某污水处理厂全年采集二氧化氯消毒前后的最终出水样本。分别采用微孔膜过滤和核酸吸附颗粒收集细菌和细胞外核酸。通过定量实时聚合酶链反应（qPCR）共检测到9种SARGs。结果显示，样本中细胞内和细胞外的 、 以及 均能被阳性检测到。总体而言，ClO消毒提高了胞内SARGs（iSARGs）的相对丰度（<0.05），呈现季节性模式，在春季、夏季和秋季增加。春季时提升最为明显，丰度增至两倍。冬季时，无论是细胞内还是细胞外均未检测到阳性的SARGs。ClO消毒前后胞外SARGs（eSARGs）浓度无显著变化。因此，ClO消毒无法有效去除污水处理厂最终出水中的iSARGs和eSARGs。
