Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Division of Occupational and Environmental Diseases, Department of Disease Control, Ministry of Public Health, Nonthaburi 11000, Thailand.
Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand.
Bioelectrochemistry. 2024 Dec;160:108780. doi: 10.1016/j.bioelechem.2024.108780. Epub 2024 Jul 14.
To advance cervical cancer diagnostics, we propose a state-of-the-art label-free electrochemical immunosensor designed for the simultaneous detection of multiple biomarker proteins (p16, p53, and Ki67). This immunosensor is constructed using a polyethyleneimine-coated gold nanoparticles/2D tungsten disulfide/graphene oxide (PEI-AuNPs/2D WS/GO) composite-modified three-screen-printed carbon electrode (3SPCE) array. The 2D WS/GO hybrid provides a large specific surface area for supporting well-dispersed PEI-AuNPs and adsorbed redox-active species, enhancing overall performance. The PEI-AuNPs-decorated 2D WS/GO composite not only improves electrode conductivity but also increases the antibody loading capacity. Redox-active species, including Cd ions, 2,3-diaminophenazine (DAP), and methylene blue (MB), serve as distinct signaling compounds to quantitatively detect the cervical cancer biomarkers p16, p53, and Ki67, respectively. Additionally, the immunosensor demonstrates the detection with high sensitivity, good storage stability, high selectivity, and acceptable reproducibility. This immunosensor demonstrates a good linear relationship with the logarithm of protein concentrations. Additionally, the immunosensor also demonstrates high sensitivity, good storage stability, high selectivity, and acceptable reproducibility. Our promising results and the successful application of the immunosensor in detecting three tumor markers in human serum highlight its potential for clinical diagnosis of cervical cancer.
为了推进宫颈癌诊断，我们提出了一种基于无标记电化学免疫传感器，用于同时检测多种生物标志物蛋白（p16、p53 和 Ki67）。该免疫传感器采用聚乙烯亚胺（PEI）包覆的金纳米粒子/二维二硫化钨/氧化石墨烯（PEI-AuNPs/2D WS/GO）复合修饰的三电极印刷碳电极（3SPCE）阵列构建。二维 WS/GO 杂化材料提供了较大的比表面积，用于支撑良好分散的 PEI-AuNPs 和吸附的氧化还原活性物质，从而提高了整体性能。PEI-AuNPs 修饰的二维 WS/GO 复合材料不仅提高了电极的导电性，而且增加了抗体的负载能力。氧化还原活性物质，包括 Cd 离子、2,3-二氨基吩嗪（DAP）和亚甲基蓝（MB），分别作为独特的信号化合物，用于定量检测宫颈癌生物标志物 p16、p53 和 Ki67。此外，该免疫传感器具有高灵敏度、良好的储存稳定性、高选择性和可接受的重现性。该免疫传感器与蛋白质浓度的对数呈良好的线性关系。此外，该免疫传感器还表现出高灵敏度、良好的储存稳定性、高选择性和可接受的重现性。我们的研究结果表明，该免疫传感器具有良好的应用前景，可用于临床宫颈癌的诊断。
