Thrombosis and Healthy Aging Research Center, Department of Clinical Biochemistry and Immunohaematology, Faculty of Health Sciences, University of Talca, Talca 3460000, Chile.
Thrombosis and Healthy Aging Research Center, Department of Clinical Biochemistry and Immunohaematology, Faculty of Health Sciences, University of Talca, Talca 3460000, Chile.
Biomed Pharmacother. 2024 Aug;177:117154. doi: 10.1016/j.biopha.2024.117154. Epub 2024 Jul 16.
This study investigates the antiplatelet properties of tomato pulp to combat cardiovascular diseases. Notably, it examines the formation of nitrated fatty acids (NO-FA) in tomato pomace, renowned for its potential antiplatelet effects. Through diverse assays, including tandem mass spectrometry, microplate-based platelet aggregation, and flow cytometry, the research identifies NO-OA, NO-LA, and NO-LnA as pivotal antiplatelet compounds. It demonstrates the concentration-dependent antiplatelet effects of nitrated tomato pomace against thrombin receptor activator peptide 6 (TRAP-6) and collagen-induced platelet activation, alongside the modulation of platelet activation markers. Additionally, synergistic effects were observed with nitrated tomato pomace extracts. The findings suggest therapeutic potential for NO-FA derived from tomato pomace in preventing blood clot formation, with nitrated extracts exhibiting superior efficacy compared to non-nitrated ones. This research highlights the promising role of natural products, such as tomato pomace, in mitigating cardiovascular risks and proposes novel strategies for population health enhancement and cardiovascular disease management.
本研究旨在探讨番茄浆的抗血小板特性，以防治心血管疾病。特别是，研究考察了番茄渣中产生的硝化脂肪酸（NO-FA），这是一种具有潜在抗血小板作用的物质。通过串联质谱、基于微孔板的血小板聚集和流式细胞术等多种检测方法，研究发现 NO-OA、NO-LA 和 NO-LnA 是关键的抗血小板化合物。研究表明，硝化番茄渣对凝血酶受体激活肽 6（TRAP-6）和胶原诱导的血小板激活具有浓度依赖性的抗血小板作用，同时还调节了血小板激活标志物。此外，还观察到硝化番茄渣提取物具有协同作用。研究结果表明，源自番茄渣的 NO-FA 具有预防血栓形成的治疗潜力，硝化提取物比非硝化提取物具有更优异的功效。本研究强调了天然产物（如番茄渣）在减轻心血管风险方面的潜在作用，并为提高人群健康水平和管理心血管疾病提出了新的策略。
