National Key Laboratory of Veterinary Public Health Security, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Key Laboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture & Beijing Key Laboratory of Animal Genetic Improvement, China Agricultural University, Beijing 100193 China.
Vet Parasitol. 2024 Oct;331:110245. doi: 10.1016/j.vetpar.2024.110245. Epub 2024 Jun 28.
Live anticoccidial vaccines, either formulated with unattenuated or attenuated Eimeria parasites, are powerful stimulators of chicken intestinal immunity. Little is known about the dynamics of gene expression and the corresponding biological processes of chicken responses against infection with precocious line (PL) of Eimeria parasites. In the present study, we performed a time-series transcriptomic analysis of chicken duodenum across 15 time points from 6 to 156 hours post-infection (p.i.) with PL of E. acervulina. A high-quality profile showing two distinct changes in chicken duodenum mRNA expression was generated during the infection of Eimeria. Early response revealed that activation of the chicken immune response was detectable from 6 h.p.i., prominent genes triggered during the initiation of asexual and sexual parasite growth encompass immune regulatory effects, such as interferon gamma (IFN-γ), interferon regulatory factor 1 (IRF1), and interleukin-10 (IL10). The late response was identified significantly associating with maintaining cellular structure and activating lipid metabolic pathways. These analyses provide a detailed depiction of the biological response landscape in chickens infected by the PL of E. acervulina, contributing significant insights for the investigation of the host-parasite interactions and the management of parasitic diseases.
活抗球虫疫苗，无论是用未减毒还是减毒的艾美耳球虫寄生虫配制的，都是刺激鸡肠道免疫的有力刺激物。对于鸡对早熟系（PL）艾美耳球虫寄生虫感染的反应的基因表达动态和相应生物学过程知之甚少。在本研究中，我们对感染 PL 的鸡十二指肠进行了 15 个时间点的时间序列转录组分析E. acervulina 从 6 小时到 156 小时。在艾美耳球虫感染期间，生成了一个显示鸡十二指肠 mRNA 表达有两个明显变化的高质量图谱。早期反应表明，鸡免疫反应的激活可从 6 小时检测到。在无性和有性寄生虫生长开始时触发的主要基因包括免疫调节作用，如干扰素γ（IFN-γ）、干扰素调节因子 1（IRF1）和白细胞介素 10（IL10）。晚期反应与维持细胞结构和激活脂质代谢途径显著相关。这些分析提供了鸡感染 PL 的 E. acervulina 后的生物学反应图谱的详细描述，为宿主-寄生虫相互作用的研究和寄生虫病的管理提供了重要的见解。
