National Key Laboratory of Veterinary Public Health Security, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Genes (Basel). 2024 Jun 24;15(7):831. doi: 10.3390/genes15070831.
Coccidiosis, a parasitic disease caused by single or multiple species, leads to significant economic losses in the poultry industry. The life cycle includes schizogony, gametogony, and sporogony. To investigate the dynamics of gene expression and regulatory networks during the development of , we employed time-course transcriptomics to rigorously compare the gene expression patterns between a precocious line (PL) and the wild type (WT) of . The results revealed that the PL enters into gametogony 12 h earlier than the WT, and both the PL and WT exhibited distinct clustering patterns during the development phase. A weighted gene co-expression network analysis (WGCNA) identified genes specifically expressed at four distinct developmental stages, schizogony, gametogony, sporulated oocysts, and unsporulated oocysts, clarifying the key biological processes at each stage. This study used global transcriptome profiling to elucidate molecular variations throughout the life cycle, providing critical insights into molecular characterization and valuable resources for investigating other apicomplexan parasites of public health importance.
球虫病是一种由单一或多种物种引起的寄生虫病，给家禽业造成了巨大的经济损失。其生命周期包括裂殖生殖、配子生殖和孢子生殖。为了研究在 发育过程中基因表达和调控网络的动态变化，我们采用时间序列转录组学方法，严格比较早熟系（PL）和野生型（WT）之间的基因表达模式。结果表明，PL 比 WT 提前 12 小时进入配子生殖期，PL 和 WT 在发育阶段都表现出明显的聚类模式。加权基因共表达网络分析（WGCNA）鉴定了在四个不同发育阶段（裂殖生殖、配子生殖、孢子化卵囊和未孢子化卵囊）特异性表达的基因，阐明了每个阶段的关键生物学过程。本研究利用全基因组转录组谱分析阐明了生命周期中的分子变化，为分子特征提供了重要见解，并为研究其他重要公共卫生的顶复门寄生虫提供了有价值的资源。
