鱼类干扰素 γ、δ 和 λ对 STAT1 和 STAT2 磷酸化的不同激活作用。
Different activation of STAT1 and STAT2 phosphorylation by IFNc, IFNd, and IFNh in tilapia.
机构信息
Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, and Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institute, College of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, And Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings, Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518120, China.
Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, and Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institute, College of Fishery, Guangdong Ocean University, Zhanjiang 524088, China.
出版信息
Fish Shellfish Immunol. 2024 Sep;152:109776. doi: 10.1016/j.fsi.2024.109776. Epub 2024 Jul 16.
Type I IFNs are a subset of cytokines exerting their antiviral effects mainly through the JAK-STAT signalling. Immunogenetic studies have shown that fish possess key components of IFN-JAK-STAT cascade, but the information about the distinct responses of STAT1 and STAT2 to different IFNs is rather limited in fish. Here, we identified and cloned STAT1 and STAT2 genes (named as On-STAT1 and On-STAT2) from tilapia, Oreochromis niloticus. On-STAT1 and On-STAT2 genes were detected in all orangs/tissues examined, and were rapidly induced in spleen, head kidney, and liver following the stimulation of poly(I:C). In addition, the stimulation of poly(I:C), poly(A:T), and different subgroups of recombinant IFNs could induce the expression of On-STAT1 and On-STAT2 in TA-02 cells with distinct induction levels. Importantly, On-STAT2 was rapidly phosphorylated by all three subgroups of IFNs, but the phosphorylation of On-STAT1 was only observed in IFNc- and IFNh-treated TA-02 cells, reflecting the distinct activation of STAT by different subgroups of fish IFNs. The present results thus contribute to better understanding of the JAK-STAT signalling mediated by different subgroups of IFNs in fish.
I 型干扰素是细胞因子的一个子集，主要通过 JAK-STAT 信号通路发挥其抗病毒作用。免疫遗传学研究表明，鱼类具有 IFN-JAK-STAT 级联反应的关键组成部分，但关于 STAT1 和 STAT2 对不同 IFN 的不同反应的信息在鱼类中相当有限。在这里，我们从罗非鱼（Oreochromis niloticus）中鉴定和克隆了 STAT1 和 STAT2 基因（命名为 On-STAT1 和 On-STAT2）。On-STAT1 和 On-STAT2 基因在所有检测到的器官/组织中均有检测到，在用 poly(I:C)刺激后，在脾脏、头肾和肝脏中迅速诱导。此外，poly(I:C)、poly(A:T)和不同亚群的重组 IFN 的刺激可以在 TA-02 细胞中诱导 On-STAT1 和 On-STAT2 的表达，诱导水平不同。重要的是，所有三个亚群的 IFN 均可迅速使 On-STAT2 磷酸化，但仅在 IFNc 和 IFNh 处理的 TA-02 细胞中观察到 On-STAT1 的磷酸化，反映了不同亚群鱼类 IFN 对 STAT 的不同激活。因此，这些结果有助于更好地理解鱼类不同亚群 IFN 介导的 JAK-STAT 信号通路。
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