PhD student, Department of Dental Materials and Prosthodontics, School of Dentistry of Ribeirao Preto, University of Sao Paulo (USP), Ribeirao Preto, SP, Brazil.
Adjunct Professor, Department of Dentistry, Federal University of Santa Catarina (UFSC), Florianopolis, SC, Brazil.
J Prosthet Dent. 2024 Sep;132(3):603.e1-603.e8. doi: 10.1016/j.prosdent.2024.06.019. Epub 2024 Jul 16.
The optimal disinfection protocol that controls adverse effects and promotes effective antimicrobial action on removable prostheses is unclear.
This in vitro study investigated the effect of disinfectant solutions on the biological, physical, mechanical, and chemical properties of removable prosthesis materials.
Specimens of polymethyl methacrylate (PMMA) and cobalt chromium (Co-Cr) alloy were immersed in distilled water (PMMA) or artificial saliva (Co-Cr) as the control and in 0.25% sodium hypochlorite (NaOCl0.25%), 0.5% chloramine T (CT0.5%), and 0.15% Triclosan (TR0.15%). The antibiofilm activity was evaluated by microbial load and cell metabolisms of the mixed biofilm. Physical (color change, sorption, solubility, and surface roughness), mechanical (hardness, flexural, and impact strength), and chemical (corrosion) properties were analyzed before and after simulating a 5-year immersion. Laser confocal microscopy, scanning electron microscopy (SEM), and dispersive energy spectroscopy (EDS) complemented the analyses. The data were analyzed by using the Mann-Whitney U test, Kruskal-Wallis with Dunn posttests, 1-way ANOVA, and repeated measures ANOVA (α=.05).
All solutions were effective against bacteria, but only NaOCl0.25% eliminated Candida spp. TR0.15%, and CT0.5% increased cell metabolisms. For interaction (time and solution), there was a reduction in PMMA hardness in the control and TR0.15%. Color, sorption, solubility, and flexural strength did not change. CT0.5% and TR0.15% were similar for impact resistance. CT0.5% caused the lowest roughness. NaOCl0.25% showed the greatest corrosive potential. Dark spots were seen under SEM in Co-Cr stored with NaOCl0.25% and TR0.15%. EDS indicated different proportions of oxygen, cobalt, chromium, and molybdenum.
NaOCl0.25% had the best antimicrobial action. CT0.5% and TR0.15% have potential. Hardness and roughness changes were clinically acceptable, and the other properties remained unchanged. All the solutions caused color changes. NaOCl0.25% was unsatisfactory for use with Co-Cr, CT0.5% was intermediate, and TR0.15% was suitable.
控制可摘义齿的不良反应并促进其有效抗菌作用的最佳消毒方案仍不明确。
本体外研究旨在调查消毒剂对可摘义齿材料的生物、物理、机械和化学性能的影响。
将聚甲基丙烯酸甲酯（PMMA）和钴铬合金（Co-Cr）标本分别浸入蒸馏水中（PMMA）或人工唾液中（Co-Cr）作为对照，以及 0.25%次氯酸钠（NaOCl0.25%）、0.5%氯胺 T（CT0.5%）和 0.15%三氯生（TR0.15%）中。通过混合生物膜的微生物负荷和细胞代谢来评估抗生物膜活性。在模拟 5 年浸泡之前和之后分析物理（颜色变化、吸附、溶解度和表面粗糙度）、机械（硬度、弯曲和冲击强度）和化学（腐蚀）性能。激光共聚焦显微镜、扫描电子显微镜（SEM）和能量色散光谱（EDS）补充了分析。使用曼-惠特尼 U 检验、克鲁斯卡尔-瓦利斯与邓恩事后检验、单因素方差分析和重复测量方差分析（α=.05）分析数据。
所有溶液对细菌均有效，但只有 NaOCl0.25%可消除白色念珠菌。TR0.15%和 CT0.5%增加了细胞代谢。对于（时间和溶液）的相互作用，在对照和 TR0.15%中 PMMA 的硬度降低。颜色、吸附、溶解度和弯曲强度均未改变。CT0.5%和 TR0.15%对冲击强度的影响相似。CT0.5%导致最低的粗糙度。NaOCl0.25%显示出最大的腐蚀性潜力。在 Co-Cr 中储存的 SEM 下可见黑色斑点，该 Co-Cr 分别用 NaOCl0.25%和 TR0.15%处理。EDS 表明存在不同比例的氧、钴、铬和钼。
NaOCl0.25%具有最佳的抗菌作用。CT0.5%和 TR0.15%有潜力。硬度和粗糙度的变化在临床可接受范围内，其他性能保持不变。所有溶液均导致颜色变化。NaOCl0.25%不适用于 Co-Cr，CT0.5%为中等，TR0.15%则合适。
