Second Department of Dental Implant, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, 350001, People's Republic of China.
Fujian Key Laboratory of Oral Diseases, Fujian Biological Materials Engineering and Technology Center of Stomatology, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, Fujian, 350004, People's Republic of China.
Int J Nanomedicine. 2024 Feb 8;19:1273-1285. doi: 10.2147/IJN.S436613. eCollection 2024.
To investigate the inhibition of Streptococcus mutans (S.mutans) and its biofilm by AgBr-nanoparticles (NP) @CTMAB (cetyltrimethyl-ammonium bromide) and evaluate the changes in Polymethyl methacrylate (PMMA)'s surface roughness (Ra), microhardness, and flexural strength during prolonged immersion in AgBr-NP@CTMAB for application in the denture cleaning industry.
The antibacterial activity of AgBr-NP@CTMAB against S.mutans was measured colony formation assay, OD600 and laser confocal microscopy. Changes in the specimens' values for surface roughness, microhardness, and flexural strength (MPa) were measured after immersion solutions for 180 or 360 days.
The AgBr-NP@CTMAB solution exhibited a robust antibacterial effect on planktonic S. mutans, with a minimum bactericidal concentration of 5 µg/mL. The 10 µg/mL AgBr-NP@CTMAB solution efficiently inhibited S. mutans biofilm formation. (2) No significant difference in surface roughness after immersion in AgBr-NP@CTMAB (10 µg/mL and 20 µg/mL) comparing with distilled water (P > 0.05) and Polident had significantly higher than distilled water (P < 0.05). There was a significant decrease in the surface hardness of the PMMA specimens that were immersed in the Polident compared with those in distilled water (P < 0.05). While, no significant differences in surface hardness after immersion in the AgBr-NP@CTMAB (P > 0.05). The result of flexural strength suggested that there was no statistically significant difference (P < 0.05) between AgBr-NP@CTMAB as well as Polident and water.
AgBrNP@CTMAB can efficiently inhibit the growth of plankton S.mutans and biofilm formation, without affecting the flexural strength, microhardness, or surface roughness of PMMA. Therefore, AgBrNP@CTMAB holds promise as a new denture cleaning agent.
研究 AgBr-纳米粒子（NP）@CTMAB（十六烷基三甲基溴化铵）对变形链球菌（S.mutans）的抑制作用，并评估其在延长浸入 AgBr-NP@CTMAB 期间对聚甲基丙烯酸甲酯（PMMA）表面粗糙度（Ra）、显微硬度和弯曲强度的影响，以便将其应用于义齿清洁行业。
通过平板菌落形成实验、OD600 和激光共聚焦显微镜测量 AgBr-NP@CTMAB 对 S.mutans 的抗菌活性。在浸入溶液 180 或 360 天后，测量标本表面粗糙度、显微硬度和弯曲强度（MPa）的变化。
AgBr-NP@CTMAB 溶液对浮游 S. mutans 具有强大的抗菌作用，最低杀菌浓度为 5 µg/mL。10 µg/mL 的 AgBr-NP@CTMAB 溶液可有效抑制 S. mutans 生物膜的形成。（2）与蒸馏水（P > 0.05）和 Polident 相比，浸入 AgBr-NP@CTMAB（10 µg/mL 和 20 µg/mL）后表面粗糙度无显著差异（P > 0.05），且 Polident 明显高于蒸馏水（P < 0.05）。浸入 Polident 的 PMMA 标本表面硬度明显低于蒸馏水（P < 0.05）。而浸入 AgBr-NP@CTMAB 后表面硬度无显著差异（P > 0.05）。弯曲强度的结果表明，AgBr-NP@CTMAB 与 Polident 和水之间无统计学差异（P < 0.05）。
AgBrNP@CTMAB 能有效抑制浮游 S.mutans 的生长和生物膜的形成，同时不影响 PMMA 的弯曲强度、显微硬度或表面粗糙度。因此，AgBrNP@CTMAB 有望成为一种新型义齿清洁剂。
