Waalkes Matthew R, Leathery Maegan, Peck Madeline, Barr Allison, Cunill Alexander, Hageter John, Horstick Eric J
Department of Biology Morgantown, West Virginia University, Morgantown, WV, USA.
Department of Neuroscience Morgantown, West Virginia University, Morgantown, WV, USA.
Sci Rep. 2024 Jul 17;14(1):16533. doi: 10.1038/s41598-024-67262-9.
Visual systems have evolved to discriminate between different wavelengths of light. The ability to perceive color, or specific light wavelengths, is important as color conveys crucial information about both biotic and abiotic features in the environment. Indeed, different wavelengths of light can drive distinct patterns of activity in the vertebrate brain, yet what remains incompletely understood is whether distinct wavelengths can invoke etiologically relevant behavioral changes. To address how specific wavelengths in the visible spectrum modulate behavioral performance, we use larval zebrafish and a stereotypic light-search behavior. Prior work has shown that the cessation of light triggers a transitional light-search behavior, which we use to interrogate wavelength-dependent behavioral modulation. Using 8 narrow spectrum light sources in the visible range, we demonstrate that all wavelengths induce motor parameters consistent with search behavior, yet the magnitude of search behavior is spectrum sensitive and the underlying motor parameters are modulated in distinct patterns across short, medium, and long wavelengths. However, our data also establishes that not all motor features of search are impacted by wavelength. To define how wavelength modulates search performance, we performed additional assays with alternative wavelengths, dual wavelengths, and variable intensity. Last, we also tested blind larvae to resolve which components of wavelength dependent behavioral changes potentially include signaling from non-retinal photoreception. These findings have important implications as organisms can be exposed to varying wavelengths in laboratory and natural settings and therefore impose unique behavioral outputs.
视觉系统已经进化到能够区分不同波长的光。感知颜色或特定光波长的能力很重要，因为颜色传达了有关环境中生物和非生物特征的关键信息。事实上，不同波长的光可以驱动脊椎动物大脑中不同的活动模式，但仍未完全理解的是，不同波长是否能引发病因相关的行为变化。为了研究可见光谱中的特定波长如何调节行为表现，我们使用斑马鱼幼体和一种刻板的光搜索行为。先前的研究表明，光照停止会触发一种过渡性的光搜索行为，我们利用这种行为来探究波长依赖性的行为调节。使用8个可见范围内的窄光谱光源，我们证明所有波长都会诱导与搜索行为一致的运动参数，但搜索行为的幅度对光谱敏感，并且潜在的运动参数在短、中、长波长范围内以不同模式受到调节。然而，我们的数据也表明，并非搜索行为的所有运动特征都受波长影响。为了确定波长如何调节搜索表现，我们用替代波长、双波长和可变强度进行了额外的实验。最后，我们还测试了失明的幼体，以确定波长依赖性行为变化的哪些成分可能包括来自非视网膜光感受器的信号。这些发现具有重要意义，因为生物体在实验室和自然环境中可能会暴露于不同波长下，从而产生独特的行为输出。
