豌豆潜叶蝇（双翅目：瘿蚊科）的染色体水平基因组组装

The chromosome-level genome assembly of Aphidoletes aphidimyza Rondani (Diptera: Cecidomyiidae).

作者信息

Shen Xiuxian, Jin Jianfeng, Zhang Guoqiang, Yan Bin, Yu Xiaofei, Wu Huizi, Yang Maofa, Zhang Feng

机构信息

Institute of Entomology, Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, College of Agriculture, Guizhou University, Guiyang, 550025, China.

Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.

出版信息

Sci Data. 2024 Jul 17;11(1):785. doi: 10.1038/s41597-024-03614-4.

DOI:10.1038/s41597-024-03614-4

PMID:39019956

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11255235/

Abstract

Aphidoletes aphidimyza is widely recognized as an effective predator of aphids in agricultural systems. However, there is limited understanding of its predation mechanisms. In this study, we generated a high-quality chromosome level of the A. aphidimyza genome by combining PacBio, Illumina, and Hi-C data. The genome has a size of 192.08 Mb, with a scaffold N50 size of 46.85 Mb, and 99.08% (190.35 Mb) of the assembly is located on four chromosomes. The BUSCO analysis of our assembly indicates a completeness of 97.8% (n = 1,367), including 1,307 (95.6%) single-copy BUSCOs and 30 (2.2%) duplicated BUSCOs. Additionally, we annotated a total of 13,073 protein-coding genes, 18.43% (35.40 Mb) repetitive elements, and 376 non-coding RNAs. Our study is the first time to report the chromosome-scale genome for the species of A. aphidimyza. It provides a valuable genomic resource for the molecular study of A. aphidimyza.

摘要

食蚜瘿蚊被广泛认为是农业系统中蚜虫的有效捕食者。然而，对其捕食机制的了解有限。在本研究中，我们通过整合PacBio、Illumina和Hi-C数据，生成了高质量的食蚜瘿蚊染色体水平基因组。该基因组大小为192.08 Mb，支架N50大小为46.85 Mb，组装的99.08%（190.35 Mb）位于四条染色体上。我们组装的BUSCO分析表明完整性为97.8%（n = 1,367），包括1,307个（95.6%）单拷贝BUSCO和30个（2.2%）重复BUSCO。此外，我们总共注释了13,073个蛋白质编码基因、18.43%（35.40 Mb）的重复元件和376个非编码RNA。我们的研究首次报道了食蚜瘿蚊物种的染色体规模基因组。它为食蚜瘿蚊的分子研究提供了有价值的基因组资源。