Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
Qianjiangyuan National Park, Kaihua, Zhejiang, 324300, China.
Sci Data. 2024 Feb 17;11(1):218. doi: 10.1038/s41597-024-03016-6.
As an important forestry pest, Coronaproctus castanopsis (Monophlebidae) has caused serious damage to the globally valuable Gutianshan ecosystem, China. In this study, we assembled the first chromosome-level genome of the female specimen of C. castanopsis by merging BGI reads, HiFi long reads and Hi-C data. The assembled genome size is 700.81 Mb, with a scaffold N50 size of 273.84 Mb and a contig N50 size of 12.37 Mb. Hi-C scaffolding assigned 98.32% (689.03 Mb) of C. Castanopsis genome to three chromosomes. The BUSCO analysis (n = 1,367) showed a completeness of 91.2%, comprising 89.2% of single-copy BUSCOs and 2.0% of multicopy BUSCOs. The mapping ratio of BGI, second-generation RNA, third-generation RNA and HiFi reads are 97.84%, 96.15%, 97.96%, and 99.33%, respectively. We also identified 64.97% (455.3 Mb) repetitive elements, 1,373 non-coding RNAs and 10,542 protein-coding genes. This study assembled a high-quality genome of C. castanopsis, which accumulated valuable molecular data for scale insects.
作为一种重要的林业害虫，栗山宽盾蝽（Monophlebidae）对中国全球重要的古田山生态系统造成了严重破坏。在这项研究中，我们通过合并 BGI reads、HiFi 长读长和 Hi-C 数据，组装了栗山宽盾蝽雌性标本的首个染色体水平基因组。组装的基因组大小为 700.81Mb，支架 N50 大小为 273.84Mb，拼接 N50 大小为 12.37Mb。Hi-C 支架将栗山宽盾蝽基因组的 98.32%（689.03Mb）分配到三个染色体上。BUSCO 分析（n=1,367）显示完整性为 91.2%，包括 89.2%的单拷贝 BUSCO 和 2.0%的多拷贝 BUSCO。BGI、第二代 RNA、第三代 RNA 和 HiFi 读取的映射率分别为 97.84%、96.15%、97.96%和 99.33%。我们还鉴定了 64.97%（455.3Mb）重复元件、1,373 个非编码 RNA 和 10,542 个蛋白质编码基因。这项研究组装了栗山宽盾蝽的高质量基因组，为鳞翅目昆虫积累了有价值的分子数据。
