Krambrich Janina, Nguyen-Tien Thang, Pham-Thanh Long, Dang-Xuan Sinh, Andersson Ella, Höller Patrick, Vu Duoc Trong, Tran Son Hai, Vu Lieu Thi, Akaberi Dario, Ling Jiaxin, Pettersson John H-O, Hesson Jenny C, Lindahl Johanna F, Lundkvist Åke
Department of Medical Biochemistry and Microbiology, Zoonosis Science Center (ZSC), Uppsala University, Uppsala, Sweden.
International Livestock Research Institute, Hanoi, Vietnam.
Sci Rep. 2024 Jul 17;14(1):16573. doi: 10.1038/s41598-024-67438-3.
Arboviruses transmitted by mosquitoes, including Japanese encephalitis virus (JEV), present a substantial global health threat. JEV is transmitted by mosquitoes in the genus Culex, which are common in both urban and rural areas in Vietnam. In 2020, we conducted a 1-year survey of Culex mosquito abundance in urban, suburban, and peri-urban areas of Hanoi using CDC-light traps. Mosquitoes were identified to species and sorted into pools based on species, sex, and trap location. The mosquito pools were also investigated by RT-qPCR for detection of JEV. In total, 4829 mosquitoes were collected over a total of 455 trap-nights, across 13 months. Collected mosquitoes included Culex, Aedes, Anopheles, and Mansonia species. Culex mosquitoes, primarily Cx. quinquefasciatus, predominated, especially in peri-urban areas. Most Culex mosquitoes were caught in the early months of the year. The distribution and abundance of mosquitoes exhibited variations across urban, suburban, and peri-urban sites, emphasizing the influence of environmental factors such as degree of urbanization, temperature and humidity on Culex abundance. No JEV was detected in the mosquito pools. This study establishes baseline knowledge of Culex abundance and temporal variation, which is crucial for understanding the potential for JEV transmission in Hanoi.
由蚊子传播的虫媒病毒，包括日本脑炎病毒（JEV），对全球健康构成重大威胁。JEV由库蚊属蚊子传播，这种蚊子在越南城乡地区都很常见。2020年，我们使用疾控中心诱蚊灯在河内的城市、郊区和城郊地区进行了为期1年的库蚊数量调查。蚊子被鉴定到物种，并根据物种、性别和诱捕地点分类成组。还通过逆转录定量聚合酶链反应（RT-qPCR）对蚊组进行检测以发现JEV。在13个月的时间里，总共455个诱捕夜共捕获4829只蚊子。捕获的蚊子包括库蚊、伊蚊、按蚊和曼蚊属物种。库蚊，主要是致倦库蚊，占主导地位，尤其是在城郊地区。大多数库蚊在一年的头几个月捕获。蚊子的分布和数量在城市、郊区和城郊地点存在差异，这突出了城市化程度、温度和湿度等环境因素对库蚊数量的影响。在蚊组中未检测到JEV。本研究建立了库蚊数量及其时间变化的基线知识，这对于了解河内JEV传播的可能性至关重要。
