Zak K P, Gruzov M A, Khomenko B M, Butenko G M, Komissarenko S V
Biull Eksp Biol Med. 1985 Oct;100(10):494-6.
The ultrastructure of different human blood T-lymphocyte subpopulations was studied by immunoelectron microscopy using "Orthoclone" monoclonal antibodies (OKT-3, OKT-4 and OKT-8) and peroxidase-antiperoxidase (PAP) complex. It was shown that T-lymphocyte subpopulations, distinct in their surface markers and function, possessed some specific features of submicroscopic structure. OKT-4+ cells (helper/inductor) were characterized by small size, high nucleus/cytoplasm ratio and few cytoplasmic organelles. OKT-8+ cells (suppressor/killer) were larger, had low nucleus/cytoplasm ratio and bean-like nucleus, with their cytoplasm containing numerous mitochondria, well-developed Golgi complex and osmiophilic granules.
利用“Orthoclone”单克隆抗体（OKT - 3、OKT - 4和OKT - 8）及过氧化物酶 - 抗过氧化物酶（PAP）复合物，通过免疫电子显微镜研究了不同人类血液T淋巴细胞亚群的超微结构。结果表明，T淋巴细胞亚群在表面标志物和功能上有所不同，具有一些亚微观结构的特定特征。OKT - 4 +细胞（辅助/诱导细胞）的特点是体积小、核质比高且细胞质细胞器少。OKT - 8 +细胞（抑制/杀伤细胞）较大，核质比低且核呈豆状，其细胞质含有大量线粒体、发达的高尔基体复合体和嗜锇颗粒。
