Živković Lada, Pirković Andrea, Topalović Dijana, Borozan Sunčica, Bajić Vladan, Srećković Vesna Dimitrijević, Djelić Ninoslav, Petrović Hristina, Milić Mirta, Spremo-Potparević Biljana
Department of Pathobiology, Faculty of Pharmacy, University of Belgrade, 11000, Belgrade, Serbia.
Department for Biology of Reproduction, University of Belgrade, Institute for Application of Nuclear Energy, 11000, Belgrade, Serbia.
Mutagenesis. 2024 Nov 2;39(6):318-326. doi: 10.1093/mutage/geae017.
Systemic oxidative stress stemming from increased free radical production and reduced antioxidant capacity are common characteristics of obese individuals. Using hydrogen peroxide (H2O2) to induce DNA damage in vitro, in peripheral blood mononuclear cells (PBMCs) from obese subjects and controls, the DNA protective ability of dihidroqercetin (DHQ) and biochaga (B) alone or in combination, were evaluated. The effects of DHQ and B were estimated under two experimental conditions: pre-treatment, where cells were pre-incubated with the substances prior to H2O2 exposure; and post-treatment when cells were first exposed to H2 H2O2, and further treated with the compounds. DNA damage was evaluated using the comet assay. The results of pre- and post-treatment showed a significant decrease in DNA damage produced by H2O2 in the obese group. This decrease was not significant in control group probably due to a small number of subjects in this pilot study. More prominent attenuation was noted in the pre-treatment with DHQ (250 μg/ml). Analysis of antioxidant properties revealed that DHQ's remarkable reducing power, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, and potent∙OH scavenging properties may contribute to strong attenuation of H2O2-induced DNA damage. Also, B showed strong reducing power, DPPH, and ∙OH scavenging ability, while reducing power and DPPH scavenger effects were increased in the presence of DHQ. Conclusively, DHQ and B may reduce H2O2-induced DNA damage in PBMCs from obese subjects when challenged in vitro, and could be valuable tools in future research against oxidative damage-related conditions.
自由基产生增加和抗氧化能力降低所导致的全身性氧化应激是肥胖个体的常见特征。利用过氧化氢（H2O2）在体外诱导肥胖受试者和对照组外周血单核细胞（PBMCs）的DNA损伤，评估二氢槲皮素（DHQ）和桦褐孔菌（B）单独或联合使用时的DNA保护能力。在两种实验条件下评估DHQ和B的效果：预处理，即细胞在暴露于H2O2之前先用这些物质预孵育；后处理，即细胞先暴露于H2O2，然后再用这些化合物处理。使用彗星试验评估DNA损伤。预处理和后处理的结果显示，肥胖组中H2O2产生的DNA损伤显著减少。在对照组中这种减少不显著，可能是由于这项初步研究中的受试者数量较少。在使用DHQ（250μg/ml）进行预处理时，观察到更显著的衰减。对抗氧化特性的分析表明，DHQ显著的还原能力、1,1-二苯基-2-苦基肼（DPPH）清除活性和强大的∙OH清除特性可能有助于强烈减轻H2O2诱导的DNA损伤。此外，B显示出强大的还原能力、DPPH和∙OH清除能力，而在存在DHQ的情况下，还原能力和DPPH清除剂效果增强。总之，在体外受到挑战时，DHQ和B可能会减少肥胖受试者PBMCs中H2O2诱导的DNA损伤，并且可能成为未来研究对抗氧化损伤相关病症的有价值工具。
