Živković Lada, Bajić Vladan, Bruić Marija, Borozan Sunčica, Popić Kristina, Topalović Dijana, Santibanez Juan, Spremo-Potparević Biljana
Center for Biological Research, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia.
Laboratory for Radiobiology and Molecular Genetics, Institute for Nuclear Research "Vinca", University of Belgrade, Mike Petrovića Alasa 12-14, 11000 Belgrade, Serbia.
Mutat Res Genet Toxicol Environ Mutagen. 2019 Sep;845:403078. doi: 10.1016/j.mrgentox.2019.06.008. Epub 2019 Aug 1.
Immune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against HO induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 μg/mL, while in posttreatment it was the concentration of 250 μg/mL. Kinetics of attenuation of HO induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15-60 min), reaching the greatest reduction after 15 and 45 min. Remarkable ·OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cell's repair machinery. Our results suggest that IA displayed antigenotoxic and antioxidant properties. A broader investigation of its profile in biological systems is needed.
免疫辅助剂（IA）由六种药用蘑菇提取物制成：姬松茸、冬虫夏草、云芝、灵芝、杂色云芝、香菇。首次评估了IA的基因保护潜力。在预处理和后处理中，在人外周血细胞中检测到针对羟基自由基（HO）诱导的DNA损伤的显著抗遗传毒性作用。IA预处理的最有效浓度为500μg/mL，而后处理的最有效浓度为250μg/mL。用最佳浓度的IA进行后处理时，HO诱导的DNA损伤的衰减动力学表明，在所有时间段（15 - 60分钟）受损细胞数量均显著减少，在15分钟和45分钟后减少幅度最大。显著的羟基自由基清除特性和适度的还原能力，以及适度的二苯基苦味酰基自由基（DPPH）清除活性，可能是在暴露于IA 15分钟后DNA损伤大幅减轻的原因，而45分钟后DNA损伤的减少可能是细胞修复机制额外受到刺激的结果。我们的结果表明，IA具有抗遗传毒性和抗氧化特性。需要对其在生物系统中的概况进行更广泛的研究。
