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在实验感染寨卡病毒的BALB/c小鼠中出现无临床表现的睾丸损伤。

Testicular damage without clinical manifestations in BALB/c mice experimentally infected with Zika virus.

作者信息

Bandeira Derick Mendes, Rasinhas Arthur da Costa, Leonardo Raphael, da Silva Marcos Alexandre Nunes, Araujo Eduarda Lima, Trindade Gisela Freitas, Santos Renata Tourinho, Mendes Ygara da Silva, Barth Ortrud Monika, Barreto-Vieira Debora Ferreira

机构信息

Laboratório de Morfologia e Morfogênese Viral, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.

Laboratório de Tecnologia Virológica, Instituto de Tecnologia em Imunobiológicos, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.

出版信息

Anim Reprod. 2024 Jul 5;21(2):e20230124. doi: 10.1590/1984-3143-AR2023-0124. eCollection 2024.

DOI:10.1590/1984-3143-AR2023-0124
PMID:39021499
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11253786/
Abstract

In 2015-2016, the Zika virus (ZIKV) caused a major epidemic in the Americas, increasing cases of microcephaly and Guillain-Barré syndrome. During this period, the discovery of ZIKV sexual transmission intensified studies on the impact of this virus on the reproductive organs. For this study, 2-month-old male BALB/c mice were infected with 1.26 x 10 PFU/mL of ZIKV in solution via the intravenous route. After three, seven, and fourteen days post-infection (DPI), blood and testicle samples were obtained to detect ZIKV RNA. The authors observed that the infected animals had slower weight gain than the control group. Viremia occurred only at 3DPI, and the ZIKV RNA was detected in one testis sample at 7DPI. The histopathological analysis of this organ revealed intense disorganization of the seminiferous tubules' structure, inflammatory infiltrate, necrosis, hemorrhage, fluid accumulation, congestion of blood vessels, and reduced sperm count. Ultrastructural analysis showed nuclear changes in tubule cells, activation of interstitial cells, and morphological changes in spermatozoa, in addition to fragmentation and decreased electron density of the genetic material of these cells. Thus, despite causing predominantly asymptomatic infections, ZIKV can cause significant subclinical and transient damage, including to male reproductive organs.

摘要

2015年至2016年期间，寨卡病毒（ZIKV）在美洲引发了一场大规模疫情，导致小头畸形和吉兰-巴雷综合征病例增加。在此期间，寨卡病毒性传播的发现加强了对该病毒对生殖器官影响的研究。在本研究中，2月龄雄性BALB/c小鼠通过静脉途径感染了溶液中1.26×10 PFU/mL的寨卡病毒。在感染后3天、7天和14天（DPI），采集血液和睾丸样本以检测寨卡病毒RNA。作者观察到，感染动物的体重增加比对照组慢。病毒血症仅在3DPI时出现，在7DPI时在一个睾丸样本中检测到寨卡病毒RNA。该器官的组织病理学分析显示，生精小管结构严重紊乱、炎性浸润、坏死、出血、积液、血管充血以及精子数量减少。超微结构分析显示，除了这些细胞的遗传物质碎片化和电子密度降低外，小管细胞还出现核变化、间质细胞活化以及精子形态变化。因此，尽管寨卡病毒主要引起无症状感染，但它可导致显著的亚临床和短暂性损害，包括对雄性生殖器官的损害。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1858/11253786/1536664fc8da/1984-3143-ar-21-2-e20230124-gf08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1858/11253786/cfb27fbd31c2/1984-3143-ar-21-2-e20230124-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1858/11253786/e8ac9386b8fc/1984-3143-ar-21-2-e20230124-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1858/11253786/b44f22015bc5/1984-3143-ar-21-2-e20230124-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1858/11253786/3f32043ff935/1984-3143-ar-21-2-e20230124-gf04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1858/11253786/a901106c6977/1984-3143-ar-21-2-e20230124-gf05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1858/11253786/2aff687aaccd/1984-3143-ar-21-2-e20230124-gf06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1858/11253786/89ce6e13c475/1984-3143-ar-21-2-e20230124-gf07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1858/11253786/1536664fc8da/1984-3143-ar-21-2-e20230124-gf08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1858/11253786/cfb27fbd31c2/1984-3143-ar-21-2-e20230124-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1858/11253786/e8ac9386b8fc/1984-3143-ar-21-2-e20230124-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1858/11253786/b44f22015bc5/1984-3143-ar-21-2-e20230124-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1858/11253786/3f32043ff935/1984-3143-ar-21-2-e20230124-gf04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1858/11253786/a901106c6977/1984-3143-ar-21-2-e20230124-gf05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1858/11253786/2aff687aaccd/1984-3143-ar-21-2-e20230124-gf06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1858/11253786/89ce6e13c475/1984-3143-ar-21-2-e20230124-gf07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1858/11253786/1536664fc8da/1984-3143-ar-21-2-e20230124-gf08.jpg

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Can J Microbiol. 2020 Feb;66(2):87-98. doi: 10.1139/cjm-2019-0331. Epub 2019 Nov 4.
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Zika Virus Infection in Tupaia belangeri Causes Dermatological Manifestations and Confers Protection against Secondary Infection.食蟹猕猴感染 Zika 病毒可引起皮肤病变并提供针对二次感染的保护。
J Virol. 2019 Apr 3;93(8). doi: 10.1128/JVI.01982-18. Print 2019 Apr 15.
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