Moar Kareena, Brahma Mettle, Pant Anuja, Maruthi Mulaka, Maurya Pawan Kumar
Department of Biochemistry, Central University of Haryana Mahendergarh 123031, India.
Int J Biochem Mol Biol. 2024 Jun 15;15(3):51-59. doi: 10.62347/BMKT5441. eCollection 2024.
Human epidemiological studies have shown that diets rich in plant polyphenols have beneficial effects on various diseases including cancer. Epigallocatechin Gallate, a flavonoid polyphenol molecule, has been shown to be both chemotherapeutic and chemo-preventive in the treatment of several forms of cancer, including lung cancer. 80% of cancers of the lungs are non-small cell lung cancers.
The study was carried out to see the effects of Epigallocatechin Gallate in non-small cell lung cancer cells (A549) using in-vitro studies.
Cell Viability Assay was performed using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. Wound Healing assay was also performed at different concentrations of the compound. Dexamethasone and Doxorubicin, the drugs with anti-cancer properties served as control. A549 cell lines were used.
In the current study, it was demonstrated using Cell viability assay and Wound Healing assay that Epigallocatechin gallate exhibits anti-proliferative activity on A549 lung cancer cells and inhibits cancer cell proliferation in a concentration and time-dependent manner. It was observed that Epigallocatechin gallate (P = 0.0016, P = 0.0018) could significantly inhibit the growth of lung cancer cells with IC50 values 60.55 ± 1.0 μM. The result of wound Healing assay suggests that Epigallocatechin gallate can inhibit the proliferation and migration of A549 cells with concentrations near or higher to 50 μM.
Epigallocatechin gallate's protective effect has been shown in A549 lung adenocarcinoma cells in a time and dose-dependent manner. This suggests the implication of Epigallocatechin gallate for the prevention and therapy for lung cancer.
人类流行病学研究表明，富含植物多酚的饮食对包括癌症在内的各种疾病具有有益影响。表没食子儿茶素没食子酸酯是一种黄酮类多酚分子，已被证明在治疗包括肺癌在内的多种癌症形式中具有化疗和化学预防作用。80%的肺癌为非小细胞肺癌。
本研究通过体外研究观察表没食子儿茶素没食子酸酯对非小细胞肺癌细胞（A549）的影响。
使用MTT（3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐）法进行细胞活力测定。还在不同浓度的该化合物下进行了伤口愈合试验。具有抗癌特性的药物地塞米松和阿霉素作为对照。使用A549细胞系。
在本研究中，通过细胞活力测定和伤口愈合试验证明，表没食子儿茶素没食子酸酯对A549肺癌细胞具有抗增殖活性，并以浓度和时间依赖性方式抑制癌细胞增殖。观察到表没食子儿茶素没食子酸酯（P = 0.0016，P = 0.0018）可显著抑制肺癌细胞生长，IC50值为60.55±1.0μM。伤口愈合试验结果表明，表没食子儿茶素没食子酸酯在浓度接近或高于50μM时可抑制A549细胞的增殖和迁移。
表没食子儿茶素没食子酸酯在A549肺腺癌细胞中呈现出时间和剂量依赖性的保护作用。这表明表没食子儿茶素没食子酸酯在肺癌的预防和治疗中具有潜在应用价值。
