General Department of Oncology, Jiangxi Cancer Hospital, The Second Affiliated Hospital of Nanchang Medical College, Jiangxi Clinical Research Center for Cancer, Nanchang, 330029, Jiangxi, People's Republic of China.
Department of Abdominal Tumor Surgery, Jiangxi Cancer Hospital, The Second Affiliated Hospital of Nanchang Medical College, Jiangxi Clinical Research Center for Cancer, Nanchang, 330029, Jiangxi, People's Republic of China.
Clin Transl Oncol. 2022 Nov;24(11):2210-2221. doi: 10.1007/s12094-022-02882-z. Epub 2022 Jul 17.
To investigate the effects of resveratrol (RSVL) on epithelial-mesenchymal transition (EMT) and biological behaviors of gastric cancer cells.
SGC-7901 cells were treated with RSVL, followed by TGF-β1 treatment for induction of EMT. Cell proliferation was tested by MTT assay, migration and invasion by Transwell and scratch assays, and Hippo-YAP signaling pathway activation by immunofluorescence. The RNA and protein expressions of E-cadherin, Vimentin, N-cadherin, and Snail were detected by qPCR and Western blot. A tumor model was constructed to examine the effect of RSVL on gastric tumor growth.
RSVL inhibited the migration, invasion, and growth of gastric cancer cells in concentration- and time-dependent manners. RSVL inhibited TGF-β1-induced EMT of gastric cancer cells, which might relate to inactivation of the Hippo-YAP pathway. In the mouse tumor model, RSVL inhibited the EMT process by suppressing the Hippo-YAP pathway.
RSVL inhibited EMT of gastric cancer cells probably by weakening the Hippo-YAP signaling pathway.
研究白藜芦醇（RSVL）对胃癌细胞上皮-间质转化（EMT）和生物学行为的影响。
用 RSVL 处理 SGC-7901 细胞，然后用 TGF-β1 处理诱导 EMT。通过 MTT 检测细胞增殖，通过 Transwell 和划痕实验检测迁移和侵袭，通过免疫荧光检测 Hippo-YAP 信号通路的激活。通过 qPCR 和 Western blot 检测 E-钙黏蛋白、波形蛋白、N-钙黏蛋白和 Snail 的 RNA 和蛋白表达。构建肿瘤模型以检测 RSVL 对胃肿瘤生长的影响。
RSVL 呈浓度和时间依赖性抑制胃癌细胞的迁移、侵袭和生长。RSVL 抑制 TGF-β1 诱导的胃癌细胞 EMT，这可能与 Hippo-YAP 通路失活有关。在小鼠肿瘤模型中，RSVL 通过抑制 Hippo-YAP 通路抑制 EMT 过程。
RSVL 可能通过削弱 Hippo-YAP 信号通路抑制胃癌细胞的 EMT。
