Rostock R A, Kopher K A, Bauer T W, Klein J L
Cancer Drug Deliv. 1985 Spring;2(2):139-45. doi: 10.1089/cdd.1985.2.139.
The effect of tumor size, vascularity, ferritin content and the amount of injected 131I-antiferritin on tumor localization was studied in four hepatoma models with varying growth rates, histology, vascularity, and ferritin content. Separate groups of 12 animals with the H-4-II-E, 7800, 7777, and 3924A rat hepatomas with less than 2 g or greater than 2 g tumors were injected with escalating doses of 131I-antiferritin or 131I nonspecific IgG (control). Tumor vascularity was measured by 51Cr-labeled erythrocyte injection, ferritin content of tumors by radioimmunoassay and immunoperoxidase staining, and the histological location of 131I-antiferritin by autoradiography. 131I-antiferritin specifically localized in the H-4-II-E and 7800 models and correlated with the tumor size, vascular content, and amount of injected antiferritin. No localization took place in the 7777 or 3924A tumors despite the presence of ferritin in these models. The only factor that correlated with localization in the models was vascularity. The vascularity of 3924A and 7777 tumors was significantly reduced in comparison to the H-4-II-E and 7800 tumors. The dependence of targeting on vascularity was demonstrated with autoradiography as well. These findings indicate the correlation of vascularity and tumor localization with 131I-antiferritin.
在四种生长速率、组织学、血管分布和铁蛋白含量各异的肝癌模型中，研究了肿瘤大小、血管分布、铁蛋白含量以及注射的131I-抗铁蛋白量对肿瘤定位的影响。将12只患有H-4-II-E、7800、7777和3924A大鼠肝癌且肿瘤小于2g或大于2g的动物分成不同组，分别注射递增剂量的131I-抗铁蛋白或131I非特异性IgG（对照）。通过注射51Cr标记的红细胞测量肿瘤血管分布，用放射免疫测定法和免疫过氧化物酶染色法测定肿瘤的铁蛋白含量，并用放射自显影法确定131I-抗铁蛋白的组织学定位。131I-抗铁蛋白特异性定位于H-4-II-E和7800模型中，且与肿瘤大小、血管含量和注射的抗铁蛋白量相关。尽管7777或3924A肿瘤模型中存在铁蛋白，但未发生定位。在这些模型中，与定位相关的唯一因素是血管分布。与H-4-II-E和7800肿瘤相比，3924A和7777肿瘤的血管分布明显减少。放射自显影也证明了靶向对血管分布的依赖性。这些发现表明血管分布与131I-抗铁蛋白的肿瘤定位之间存在相关性。
