Msirikale J S, Klein J L, Schroeder J, Order S E
Department of Radiation Oncology, Johns Hopkins Hospital, Baltimore, MD 21205.
Int J Radiat Oncol Biol Phys. 1987 Dec;13(12):1839-44. doi: 10.1016/0360-3016(87)90349-x.
Recent clinical observations led to the use of external radiation to increase tumor targeting by radiolabelled 131-I antiferritin. Examination of increased uptake of 131-I labelled antiferritin following external radiation was carried out in syngeneic implanted hepatomas (H4IIE, 3924A, 7800, and 7777). Exposure to 10 Gy increased the tumor: liver uptake ratio from 1.55 to 1.86 for H4IIE; from 1.56 to 2.0 for 7800; from 1.34 to 1.97 for 7777; and from 1.05 to 1.19 for 3924A. The pattern of uptake varied among the different tumor types, reflecting their inherent differences in vascularity, tumor permeability, antigen density and growth rate, all of which influence antibody targeting of the tumors. When tumor and liver were irradiated, the tumor showed increased differential uptake of labelled antibody compared to normal liver. 51-Cr labelled erythrocytes were used to study the relative vascularity and blood pooling in H4IIE hepatoma and normal tissue. External radiation to the tumor did not increase the uptake of 51-Cr labelled erythrocytes in any site. These studies provide an insight into the role of external radiation as a modality that increases radiolabelled antibody targeting in hepatoma.
近期的临床观察促使人们使用外照射来增强放射性标记的131-I抗铁蛋白对肿瘤的靶向作用。在同基因植入性肝癌（H4IIE、3924A、7800和7777）中，对外照射后131-I标记抗铁蛋白摄取增加的情况进行了检查。对于H4IIE，暴露于10 Gy使肿瘤与肝脏的摄取比从1.55提高到1.86；对于7800，从1.56提高到2.0；对于7777，从1.34提高到1.97；对于3924A，从1.05提高到1.19。不同肿瘤类型之间的摄取模式各不相同，这反映了它们在血管生成、肿瘤通透性、抗原密度和生长速率方面的固有差异，所有这些因素都会影响肿瘤的抗体靶向作用。当对肿瘤和肝脏进行照射时，与正常肝脏相比，肿瘤显示出标记抗体的摄取差异增加。使用51-Cr标记的红细胞来研究H4IIE肝癌及正常组织中的相对血管生成和血液蓄积情况。对肿瘤进行外照射并未增加任何部位51-Cr标记红细胞的摄取。这些研究为外照射作为一种增强肝癌中放射性标记抗体靶向作用的方式所起的作用提供了深入了解。
