Ariyanasab Reyhane, Askari Vahid Reza, Askari Roya, Baradaran Rahimi Vafa, Hejazi Keyvan, Asadi Milad
Department of Sport Physiology, Faculty of Exercise Sciences, Hakim Sabzevari University, Sabzevar, Iran.
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran.
Heliyon. 2024 Jun 19;10(12):e33241. doi: 10.1016/j.heliyon.2024.e33241. eCollection 2024 Jun 30.
We aimed to evaluate the effects of seven weeks of aerobic exercise training and piperine on paraquat-induced lung damage. Forty-eight male Wistar rats (230 g, six-eight weeks old) were randomly divided into six groups (n = 8): sham, paraquat (5 mg/kg three times a week; intraperitoneally), paraquat + piperine (10 mg/kg/day; orally), paraquat + aerobic exercise training, paraquat + piperine + aerobic exercise training; and paraquat + vitamin E (20 mg/kg/day; orally) as a positive control. Rats were sacrificed on day 50, and both lung tissues were isolated to measure oxidative (MDA), anti-oxidative (GSH), inflammatory (TNF-α), anti-inflammatory (IL-10) markers, and histological evaluations (hematoxylin-eosin staining). The results of the present study revealed that paraquat significantly decreased body weight, GSH, GSH/MDA ratio, IL-10, and IL-10/TNF-α ratio while increasing MDA, TNF-α, and histopathological damage in lung tissue (P < 0.01 to 0.001). In contrast, treatment with all four interventions meaningfully diminished oxidative, inflammatory markers, and histopathological damage while propagating body weight, anti-oxidative and anti-inflammatory markers following the paraquat-induced lung damage (P < 0.05 to P < 0.001). Interestingly, piperine and piperine + exercise training possessed stronger protective effects against paraquat-induced lung damage than exercise training alone (P < 0.01 to 0.001). Treatment with piperine, exercise training, piperine + exercise training, and vitamin E significantly ameliorated paraquat-induced lung damage. Interestingly, the piperine and piperine + exercise training had more protective effects than other groups. Therefore, piperine and the combination of piperine and exercise training may be valuable candidates for preventing lung injuries.
我们旨在评估为期七周的有氧运动训练和胡椒碱对百草枯诱导的肺损伤的影响。48只雄性Wistar大鼠（体重230克，六周龄至八周龄）被随机分为六组（每组n = 8）：假手术组、百草枯组（每周三次，每次5毫克/千克；腹腔注射）、百草枯+胡椒碱组（每天10毫克/千克；口服）、百草枯+有氧运动训练组、百草枯+胡椒碱+有氧运动训练组；以及百草枯+维生素E组（每天20毫克/千克；口服）作为阳性对照。在第50天处死大鼠，分离双侧肺组织以测量氧化指标（丙二醛）、抗氧化指标（谷胱甘肽）、炎症指标（肿瘤坏死因子-α）、抗炎指标（白细胞介素-10），并进行组织学评估（苏木精-伊红染色）。本研究结果显示，百草枯显著降低了体重、谷胱甘肽、谷胱甘肽/丙二醛比值、白细胞介素-10以及白细胞介素-10/肿瘤坏死因子-α比值，同时增加了丙二醛、肿瘤坏死因子-α以及肺组织的组织病理学损伤（P < 0.01至0.001）。相比之下，所有四种干预措施在百草枯诱导的肺损伤后，均能显著降低氧化、炎症指标以及组织病理学损伤，同时增加体重、抗氧化和抗炎指标（P < 0.05至P < 0.001）。有趣的是，胡椒碱以及胡椒碱+运动训练对百草枯诱导的肺损伤的保护作用比单独的运动训练更强（P < 0.01至0.001）。胡椒碱、运动训练、胡椒碱+运动训练以及维生素E治疗均显著改善了百草枯诱导的肺损伤。有趣的是，胡椒碱以及胡椒碱+运动训练的保护作用比其他组更强。因此，胡椒碱以及胡椒碱与运动训练的组合可能是预防肺损伤的有价值的候选方案。
